Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice.

Kang Tan, Ya-Qian Wang, Liu Fan, Hua-Tai Xu

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kang TanLingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China. Electronic address: tankang@lglagb.ac.cn.

Ya-Qian WangLingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China.

Liu FanLingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China.

Hua-Tai XuLingang Laboratory, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, 555 Qiangye Road, Shanghai 201210, China. Electronic address: xuht@lglab.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Barcoded rabies viral tracing enables high-throughput mapping of brain-wide inputs to individual neurons while allowing integration with transcriptomic profiling. Here, we present a protocol to produce and apply barcoded rabies virus in mice for single-neuron input mapping. We describe steps to generate barcode plasmid libraries, produce barcoded rabies virus, and isolate target brain regions. We then detail procedures for preparing single-cell suspensions or bulk RNA, building barcode amplicon libraries from input regions, and performing initial analysis of the sequencing data. For complete details on the use and execution of this protocol, please refer to Tan et al.

Indexed as

DNA Barcoding, TaxonomicNeuronsRabies virusSingle-Cell AnalysisAnimalsBrainMiceBioinformaticsCell BiologyCell cultureNeuroscienceSequence analysisSequencingSingle Cell

Identifiers

PMID42068549

DOI10.1016/j.xpro.2026.104537

PMCPMC13142104

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.