ArticleActa parasitologica2026
First Molecular Detection of Echinococcus granulosus Sensu Stricto in Dogs from Istanbul's Anatolian Side: A Multi-methodological Approach.
Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
purposeCystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of Echinococcus granulosus sensu lato (s.l.). Istanbul’s large stray dog population represents a significant risk for human CE transmission. However, prevalence and genotype studies about E. granulosus s.l. in dogs remain limited. This study aimed to determine the presence and genotypes of E. granulosus s.l. in stray dogs on the Anatolian side of Istanbul using microscopy, Copro-Enzyme-Linked Immunosorbent Assay (Copro-ELISA), and molecular diagnostic methods.
methodsFecal samples (n = 110) were collected from stray dogs in seven districts. Samples were examined using modified formalin–ethyl acetate sedimentation (mFEAS). Coproantigens were detected using a commercial ELISA kit. Genomic DNA was extracted for PCR targeting the mitochondrial CO1 gene, and positive amplicons were sequenced and evaluated phylogenetically.
resultsMicroscopy revealed Taeniid-type eggs in 2 samples (1.8%) and these were confirmed molecularly. Copro-ELISA detected 7 positive samples (6.4%); however, none of these were confirmed by molecular and microscopy. Sequencing analysis revealed E. granulosus sensu stricto (s.s.) (G1 genotype) in 2 dogs from Sultanbeyli and Beykoz. Phylogenetic analysis demonstrated both isolates clustered within the E. granulosus s.s. (G1–G3) clade.
conclusionsThis study provides the first molecular confirmation of E. granulosus s.s. in stray dogs on Istanbul’s Anatolian side, showing active zoonotic transmission potential in this highly populated region. The discrepancy between Copro-ELISA and molecular/microscopic findings highlights the requirement for multi-methodological approach. Continuing prevalence studies and molecular surveillance within a One Health framework are essential to reduce the public health threat posed by CE.
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