Evidence map›Paper›PMID 42068389›Full record

ArticleBioprocess and biosystems engineering2026

Designing strategies for high-level production of peptide-N-glycosidase F (PNGase F) from Flavobacterium meningosepticum using high cell density fed-batch culture of E. coli.

Shilpa Mohanty, Babbal, Shivani Chauhan, Mohini Talwar, Shubham Sharma, Priya Maravi, Yogender Pal Khasa

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Article in Bioprocess and biosystems engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shilpa MohantyDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
BabbalDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
Shivani ChauhanDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
Mohini TalwarDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
Shubham SharmaDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
Priya MaraviDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India.
Yogender Pal KhasaDepartment of Microbiology, University of Delhi South Campus, New Delhi, 110 021, India. yogi110@gmail.com.

Funding

IoE University of Delhi Ref. No./IoE/2024-25/12/FRP
6 · The paper itself

Abstract

Protein N-glycosylation is a crucial post-translational modification that regulates cellular processes such as cell signalling, development, and autophagy. Abnormalities in protein glycosylation can manifest in life-threatening conditions, such as cancer. N-glycan analysis is crucial for determining the underlying cause of disease. The characterization of N-linked glycans is achieved through the removal of the carbohydrate moiety using deglycosylating enzymes. Peptide-N-glycosidase F (PNGase F) is a glycoamidase that hydrolyzes the amide bond in glycosamide by specifically cleaving at the innermost N-acetylglucosamine (GlcNAc). Although PNGase F has significant therapeutic applications, its widespread commercial use is limited by its high cost. This study focused on heterologous expression of Flavobacterium meningosepticum PNGase F in E. coli BL21 (DE3) and SHuffle

Indexed as

Bacterial ProteinsBatch Cell Culture TechniquesEscherichia coliFlavobacteriumPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidaseGlycosylationRecombinant ProteinsBacterial ProteinsPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidaseRecombinant ProteinsBioprocess optimizationDeglycosylationFed-batch fermentationGlycosylationPeptide-N-glycosidase F (PNGase F)RNase B

Identifiers

PMID42068389

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.