Evidence map›Paper›PMID 42067785›Full record

ArticleBMC plant biology2026

Transcriptomics and functional characterization identify CYP73A16 as a key regulator of flavonoid biosynthesis in mulberry leaves.

Rurou Long, Xueying Jin, Michael Ackah, Frank Kwarteng Amoako, Frank Kwekucher Ackah, Frank Kumi, Jianbin Li, Hao Wei, John Nelson Buah, Mengdi Zhao and 1 more

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In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Rurou Long *Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China.
Xueying Jin *Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China. jinxueying@just.edu.cn.
Michael AckahJiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China.
Frank Kwarteng AmoakoInstitute of Plant Nutrition and Soil Science, Kiel University, Hermann- Rodewald-Straße 2, Kiel, 24118, Germany.
Frank Kwekucher AckahDepartment of Crop Science, School of Agriculture, College of Agriculture and Natural Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.
Frank KumiDepartment of Crop Science, School of Agriculture, College of Agriculture and Natural Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.
Jianbin LiJiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China.
Hao WeiJiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China.
John Nelson BuahDepartment of Crop Science, School of Agriculture, College of Agriculture and Natural Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.
Mengdi ZhaoDepartment of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215011, P. R. China. mdzhao@usts.edu.cn.
Weiguo ZhaoJiangsu Key Laboratory of Sericulture Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China. wgzsri@126.com.

Funding

Key R&D Program of Guangxi AB23026066Natural Science Foundation of Jiangsu Province for Youths BK20230662
6 · The paper itself

Abstract

backgroundMulberry cultivars are well known for their flavonoid content. However, flavonoid content and the key genes involved in flavonoid biosynthesis in mulberry cultivars Zhong Shen 1 Hao (ZS) and Lv Shenzi (LSZ) are not well documented. This study aimed to analyze the flavonoid content and the key genes involved in flavonoid biosynthesis in ZS and LSZ cultivars. In this study, biochemical, transcriptomic, and functional analyses via virus-induced gene silencing (VIGS) were performed to analyze flavonoid content and key genes involved in flavonoid biosynthesis in ZS and LSZ.

resultsThe content of flavonoids in LSZ is higher than in ZS, increasing by 38.1% compared to ZS. Transcriptome analysis identified 1938 differentially expressed genes (DEGs), with 1124 downregulated and 814 upregulated, and 14 DEGs were identified as key genes significantly enriched in flavonoid biosynthesis. Transient silencing of MaCYP73A16 notably decreased not only the expression of downstream genes, including MaCYP73A16, MaLAR, MaCHS, MaCYP93B1, and MaCYP98A2, but also reduced flavonoid content, with peak silencing efficiency observed on the ninth day, underscoring the impact of silencing MaCYP73A16 on other genes involved in the flavonoid biosynthesis pathway.

conclusionThis study provides a fundamental overview of flavonoid biosynthesis in LSZ and ZS and the key genes involved, which will help facilitate a comprehensive analysis of this pathway and provide significant insight into the large-scale biosynthesis of medicinal flavonoids, thereby improving flavonoid content in these cultivars.

Indexed as

Cytochrome P-450 Enzyme SystemFlavonoidsMorusPlant LeavesPlant ProteinsGene Expression ProfilingGene Expression Regulation, PlantGene SilencingGenes, PlantTranscriptomeCytochrome P-450 Enzyme SystemFlavonoidsPlant ProteinsCYP73A16Flavonoid biosynthesisGene silencingMulberry plantTranscriptomics

Identifiers

PMID42067785
PMCPMC13348818

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