Evidence map›Paper›PMID 42065968›Full record

ArticleSTAR protocols2026

Protocol for culturing healthy primary endothelial cells isolated from research-grade human corneoscleral donor tissue.

Jeffrey M A van der Krogt, Marchien G Dallinga, Antoon J van den Bogaerdt, Ivanka J E van der Meulen, Jaap D van Buul

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jeffrey M A van der KrogtDepartment of Medical Biochemistry, Amsterdam UMC, 1105 AZ Amsterdam, the Netherlands; Department of Ophthalmology, Amsterdam UMC, 1105 AZ Amsterdam, the Netherlands. Electronic address: j.m.vanderkrogt@amsterdamumc.nl.
Marchien G DallingaDepartment of Ophthalmology, UMC Utrecht, 3584 CX Utrecht, the Netherlands.
Antoon J van den BogaerdtCornea Department, ETB-BISLIFE, 2031 CG Haarlem, the Netherlands.
Ivanka J E van der MeulenDepartment of Ophthalmology, Amsterdam UMC, 1105 AZ Amsterdam, the Netherlands.
Jaap D van BuulDepartment of Medical Biochemistry, Amsterdam UMC, 1105 AZ Amsterdam, the Netherlands; Leeuwenhoek Centre for Advanced Microscopy at Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, the Netherlands. Electronic address: j.d.vanbuul@amsterdamumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studying the function of healthy corneal endothelial cells has been challenging due to a variety of culturing strategies. Here, we present a standardized protocol for culturing primary human corneal endothelial cells, isolated from research-grade corneoscleral donor tissue, without the addition of mitotic enhancers. We describe the required enzymes, medium composition, cell density, and incubation times. Additionally, we use immunofluorescence microscopy to illustrate optimal outcomes and discuss troubleshooting procedures.

Indexed as

Cell Culture TechniquesCell SeparationEndothelial CellsEndothelium, CornealScleraCells, CulturedHumansTissue DonorsCell cultureCell isolationMicroscopy

Identifiers

PMID42065968
PMCPMC13141704

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.