ArticleSTAR protocols2026
Protocol for isolation of lipid-laden hepatocytes from high-fat diet-fed and metabolically challenged mice.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We present a protocol to isolate hepatocytes from mice fed a high-fat diet (HFD), modeling features of non-alcoholic fatty liver disease (NAFLD) and metabolically associated fatty liver disease (MAFLD). We perfuse and enzymatically digest the liver and then purify hepatocytes using a modified Percoll gradient (30%, 25%, and 10%) to retain lipid-laden cells. This protocol enables the isolation of both normal and lipid-filled hepatocytes for culture and downstream studies of drug metabolism, hepatotoxicity, and glucose or lipid metabolism in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.