ArticleChemical & biomedical imaging2026
Fast Hyperspectral and Super-Resolved Mapping of Lipid Membrane Polarity with Single-Molecule Sensitivity.
Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Imaging from Macro to Nanoscale: Multimodal Advances in Chemical and Biomedical Imaging.Chemical & biomedical imaging · 2026Review
- Deep Learning Enables Identification of Antimicrobial Peptides Through Mechanochromic Fingerprints.Angewandte Chemie (International ed. in English) · 2026Article
- Lipid Bilayer-Confined J-Aggregation Transduces Cell Membrane Mechanics into Photoacoustic Signals.Journal of the American Chemical Society · 2026Article
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Authors and funding
5 authors.
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Abstract
Cell membranes display nanoscale heterogeneity in lipid composition and organization that regulates vital biological processes yet remain challenging to resolve with conventional imaging. We introduce spectral phasor single-molecule localization microscopy (SP-SMLM), a hyperspectral and super-resolution method that combines wavefront-like optical filtering with single-molecule imaging for simultaneous spatial and spectral analysis. A lab-built three-channel imager with sine/cosine filters encodes emission spectra of single molecules into the phasor space, enabling high-throughput, high-SNR mapping of membrane polarity at sub-50 nm spatial and 15 s temporal resolutions. Through simulation, we validate that the phasor angle correlates with the spectral mean for single dye molecules. When applied to Nile red-stained COS-7 cells, SP-SMLM revealed organelle-specific polarity differences and dynamic remodeling of the lipid composition within live cells. The method's hyperspectral capability, rapid acquisition, and compatibility with 2D/3D imaging platforms position SP-SMLM as a powerful tool for studying membrane heterogeneity and dynamics in live cells.
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Registered trials
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