Evidence map›Paper›PMID 42063823›Full record

ArticleJACS Au2026

MPA-KCro: Antibody-Free Crotonylome Profiling via 2‑Mercaptophosphonic Acid Michael Addition and Titanium(IV)-Immobilized Metal Affinity Chromatography Enrichment.

Xuyang Yue, Zhuo Zhang, Enming Miao, Li Liu, Xiaoyu Song, Xiaoyan Liu, Ming Dai, Yan Wang, Mingliang Ye, Hongqiang Qin

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In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xuyang YueState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Zhuo ZhangState Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning 116024, China.
Enming MiaoState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Li LiuState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Xiaoyu SongMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Xiaoyan LiuState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Ming DaiMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Yan WangState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Mingliang YeState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.ORCID https://orcid.org/0000-0002-5872-9326
Hongqiang QinState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.ORCID https://orcid.org/0000-0002-7508-0872

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein lysine crotonylation (KCro), a metabolically linked epigenetic modification, lacks comprehensive profiling methods due to expensive, cross-reactive antibodies. Herein, we developed an antibody-free strategy, MPA-KCro, exploiting 2-mercaptophosphonic acid (2-MPA) as a bifunctional probe. The thiol group undergoes selective Michael addition with the α,β-unsaturated crotonyl moiety, while the phosphonate handle enables efficient Ti

Indexed as

2-Mercaptophosphonic acid (2-MPA)Antibody-free enrichmentChemical proteomicsHistone modificationsLysine crotonylation

Identifiers

PMID42063823
PMCPMC13126175

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.