Evidence map›Paper›PMID 42062909›Full record

ArticleBMC microbiology2026

Sustainable waste-based fermentation for enhanced β-carotene biosynthesis by Blakeslea trispora and functional evaluation of the extracted pigment.

Negin Ghazanfari, Ali Motlagh Hosseini, Fereshteh Falah, Motahareh Nasiri, Farideh Tabatabaei Yazdi, Mohamad Reza Razavi, Alireza Vasiee

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Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Negin GhazanfariDepartment of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Ali Motlagh HosseiniDepartment of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Fereshteh FalahDepartment of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Motahareh NasiriDirectorate of Research and Development of Ehyanan Iranian Company (9595), Mashhad, Iran.
Farideh Tabatabaei YazdiDepartment of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. tabatabai@um.ac.ir.
Mohamad Reza RazaviDepartment of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Alireza VasieeDepartment of Food Safety and Quality Control, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundValorization of agri-food waste as nutrient-rich substrates provides a sustainable strategy for microbial bioprocessing. Blakeslea trispora is a well-known fungal producer of natural β-carotene; however, its performance on low-cost industrial waste streams remains underexplored.

resultsIn this study, dairy sludge and soybean meal were evaluated as alternative carbon and nitrogen sources for β-carotene biosynthesis by B. trispora. Response Surface Methodology (RSM) identified an optimized medium containing 12% dairy sludge, 2% soybean meal, and 0.4% yeast extract, yielding 65 g/L biomass and 352 μg/mL β-carotene in laboratory-scale fermentation. The pigment extracted from optimized cultures was confirmed as β-carotene through HPLC, FTIR, and

conclusionThese findings demonstrate that agri-food wastes can serve as efficient substrates for fungal β-carotene production, supporting cost-effective and sustainable microbial bioprocessing. The functional properties of the extracted β-carotene further highlight its applicability as a bioactive microbial metabolite with antimicrobial, antioxidant, and anticancer potential.

Indexed as

beta CaroteneMucoralesPigments, BiologicalAnti-Infective AgentsAntioxidantsCaco-2 CellsChromatography, High Pressure LiquidCulture MediaFermentationFood Loss and WasteGlycine maxHumansMicrobial Sensitivity TestsSewageAnti-Infective AgentsAntioxidantsbeta CaroteneCulture MediaFood Loss and WastePigments, BiologicalSewageAgri-food wasteBioactive metabolitesBlakeslea trisporaMicrobial fermentationRSMβ-carotene

Identifiers

PMID42062909
PMCPMC13277224

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.