Evidence map›Paper›PMID 42062680›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy.

Vibeke S Dam, Amalie M Grønning, Benjamin B Green, Frédéric H Login, Lene N Nejsum

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Vibeke S DamDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Amalie M GrønningDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Benjamin B GreenDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Frédéric H LoginDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Lene N NejsumDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark. nejsum@clin.au.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expansion microscopy (ExM) is a powerful technique that enhances optical resolution by physically enlarging biological samples. The enlargement increases the distances between fluorophores and makes the sample transparent. Here, we present an ExM protocol optimized for tissue biopsies with 2 mm diameter and 1 mm thickness from inner medulla from perfusion-fixed rat kidneys. Besides inner medulla kidney samples, the protocol may be utilized for other tissue types with minor modifications.

Indexed as

KidneyMicroscopyAnimalsBiopsyMicroscopy, FluorescenceRatsTissue FixationExpansion microscopy (ExM)KidneyOptical resolutionProtocolTissue biopsies

Identifiers

PMID42062680

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.