Evidence map›Paper›PMID 42062676›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Multiplex Immunofluorescence Staining of Paraffin-Embedded Tissue: Coverslip Mounting of Tissue Sections Improves Z-axis Optical Resolution in Widefield Microscopy.

Gitte A Pedersen, Søren H Elsborg, Rikke Nørregaard, Lene N Nejsum

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Gitte A Pedersen *Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Søren H Elsborg *Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Rikke NørregaardDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Lene N NejsumDepartment of Clinical Medicine, Aarhus University, Aarhus, Denmark. nejsum@clin.au.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunofluorescence (IF) microscopy is essential for studying protein expression and localization. Multiplex IF enables sequential staining of the same tissue section for comprehensive analysis but often involves harsh treatments or expensive reagents that can damage tissues and reduce signal quality. Here, we present a detailed protocol for multiplex IF using indirect antibody labeling, a mild stripping buffer, and cost-effective reagents. Importantly, mounting paraffin-embedded tissue sections on gelatin-coated coverslips enhances Z-axis optical resolution in widefield imaging.

Indexed as

Fluorescent Antibody TechniqueParaffin EmbeddingStaining and LabelingAnimalsHumansMicroscopy, FluorescenceAntibody strippingEnhanced optical resolutionMultiplex immunofluorescenceParaffin-embedded tissueTissue stainingWidefield microscopy

Identifiers

PMID42062676

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.