Evidence map›Paper›PMID 42062537›Full record

ArticleBasic research in cardiology2026

Dose-dependent effects and mechanisms of exercise-like stimulation on cardiac injury and contractile function: outcomes of the MICRO-ATHLETE study.

Tom T J Luiken, Carla Cofiño-Fabres, José M Rivera-Arbeláez, Danique Snippert, Ellen J S Denessen, Sacha K Lamers, Nicoleta Cius, Koen van den Dries, Alma M A Mingels, Dick H J Thijssen and 2 more

Abstract read
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In one paragraph

Article in Basic research in cardiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tom T J LuikenDepartment of Medical BioSciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.ORCID http://orcid.org/0000-0002-2312-0414
Carla Cofiño-FabresDepartment of Applied Stem Cell Technologies, TechMed Centre, University of Twente, Enschede, The Netherlands.
José M Rivera-ArbeláezDepartment of Applied Stem Cell Technologies, TechMed Centre, University of Twente, Enschede, The Netherlands.
Danique SnippertDepartment of Applied Stem Cell Technologies, TechMed Centre, University of Twente, Enschede, The Netherlands.
Ellen J S DenessenDepartment of Clinical Chemistry, Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.
Sacha K LamersDepartment of Clinical Chemistry, Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.
Nicoleta CiusDepartment of Medical BioSciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
Koen van den DriesDepartment of Medical BioSciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
Alma M A MingelsDepartment of Clinical Chemistry, Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.
Dick H J ThijssenDepartment of Medical BioSciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
Robert C J J PassierDepartment of Applied Stem Cell Technologies, TechMed Centre, University of Twente, Enschede, The Netherlands.
Thijs M H EijsvogelsDepartment of Medical BioSciences, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands. Thijs.Eijsvogels@Radboudumc.nl.

Funding

European Research Council 101098372Nederlandse Organisatie voor Wetenschappelijk Onderzoek 024.003.001Nederlandse Organisatie voor Wetenschappelijk Onderzoek #OCENW.XS22.2.093
6 · The paper itself

Abstract

Observational studies revealed that exercise produces cardiac troponin release and cardiac fatigue in athletes. However, human in vivo studies offer limited insights into the underlying mechanisms of these potential deleterious effects. This study investigated exercise-induced cardiac troponin release, cardiac fatigue, and its potential mechanisms in a versatile in vitro Engineered Heart Tissue (EHT) model. EHTs were subjected to exercise-like electrical pulse stimulation (EL-EPS) at 2.5 Hz (150 bpm) for 2 or 4 h and compared to non-stimulated controls and doxorubicin (DOX)-exposed tissues. High-sensitive cardiac troponin T (hs-cTnT), lactate dehydrogenase (LDH), and cardiomyocyte contractile function were measured at baseline, immediately after EL-EPS, and after 20 h of recovery. Cell death was quantified by nuclear and mitochondrial DNA concentrations and tissue integrity was assessed using microscopy following exposure. Following 2 and 4 h of EL-EPS, and after DOX exposure, we found increased hs-cTnT and LDH levels and reduced contractile function compared to control. After recovery, contractile function remained impaired after 4 h EL-EPS and DOX exposure, whereas the effects were abolished after recovery for the 2 h EL-EPS group. Elevated nuclear and mitochondrial DNA release was found following DOX, but not after 2 and 4 h EL-EPS. Finally, EL-EPS was associated with the formation of actin aggregates. EL-EPS induced dose-dependent increases in hs-cTnT and LDH concentrations in conjugation with signs of cardiac fatigue, but without elevated markers of permanent cell death (i.e., mitochondrial/nuclear DNA). These findings suggest that exercise-induced elevations of cardiac biomarker concentrations are indicative of reversible cardiac injury.

Indexed as

ExerciseMyocardial ContractionMyocytes, CardiacAnimalsBiomarkersDoxorubicinElectric StimulationHumansL-Lactate DehydrogenaseTroponin TBiomarkersDoxorubicinL-Lactate DehydrogenaseTroponin TBiomarkersCardiac injuryCardiac performanceIn vitro exerciseTissue engineering

Identifiers

PMID42062537
PMCPMC13373019

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.