Evidence map›Paper›PMID 42062277›Full record

ArticleNature communications2026

Deciphering tRNA repertoires and translation coordination during mouse early embryogenesis by ORACLE-tRNAseq.

Yun Li, Xin Wang, Ruofan Huang, Haoquan Yu, You Wu, Ze Shu, Wenlin Jiang, Zheng Cao, Zongyu Yu, Hongyan Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yun Li *Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.ORCID http://orcid.org/0009-0009-4451-3194
Xin Wang *Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Ruofan Huang *Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Haoquan YuClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
You WuClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Ze ShuClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Wenlin JiangClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Zheng CaoClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Zongyu YuClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Hongyan LiSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
Rushuang YanState Key Laboratory of Cardiology and Medical Innovation Center, Department of Reproductive Medicine Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.
Yawei GaoState Key Laboratory of Cardiology and Medical Innovation Center, Department of Reproductive Medicine Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China. gaoyawei@tongji.edu.cn.ORCID http://orcid.org/0000-0001-8946-9697
Xiao-Ou ZhangClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China. zhangxiaoou@tongji.edu.cn.ORCID http://orcid.org/0000-0002-2027-1313
Yunfang ZhangClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China. zhangyunfang@tongji.edu.cn.ORCID http://orcid.org/0000-0001-9529-0431

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32170553National Natural Science Foundation of China (National Science Foundation of China) 32370869National Natural Science Foundation of China (National Science Foundation of China) 82022029National Natural Science Foundation of China (National Science Foundation of China) 82203236National Natural Science Foundation of China (National Science Foundation of China) 82371727Science and Technology Commission of Shanghai Municipality (Shanghai Municipal Science and Technology Commission) 23JC1403802
6 · The paper itself

Abstract

Translational control is vital during the maternal-to-zygotic transition (MZT), yet the landscape of embryonic transfer RNA (tRNA) pools has been difficult to explore. Here, we develop Optimized Reaction for Accurate Capture of Low-input Entities tRNA Sequencing (ORACLE-tRNAseq), enabling robust tRNA profiling from as few as five mouse oocytes. We map tRNA landscapes from oocyte to blastocyst, identifying a distinct transition to embryonic tRNA repertoires and upregulation of tRNA pseudogenes at the 4-cell stage. Integrated multi-omics analyses reveal that zygotic tRNA gene activation coincides with zygotic genome activation (ZGA) and correlates with H3K4me3 establishment and chromatin remodeling. By coupling ORACLE-tRNAseq with Ribo-seq, we demonstrate that embryonic tRNA anticodon pools coordinate with high translation-efficiency gene pools to preferentially establish the zygotic translation machinery, particularly from major ZGA onwards. Collectively, these findings provide a resource for understanding translational regulatory networks during early embryogenesis.

Indexed as

Embryonic DevelopmentProtein BiosynthesisRNA, TransferAnimalsBlastocystFemaleGene Expression Regulation, DevelopmentalHistonesMiceOocytesRibosome ProfilingSequence Analysis, RNAZygoteHistonesRNA, Transfer

Identifiers

PMID42062277
PMCPMC13338051

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.