Evidence map›Paper›PMID 42060579›Full record

ArticlePLoS genetics2026

A novel role of tRNA-derived fragments in porcine granulosa-oocyte cell communication and cuproptosis.

Linyuan Shen, Xue Zhao, Shuang Wu, Yuhang Lei, Shuang Liang, Saihao Wang, Haodong Dai, Yan Wang, Lei Chen, Ye Zhao and 4 more

Abstract read
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In one paragraph

Article in PLoS genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Linyuan ShenFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Xue ZhaoFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.ORCID https://orcid.org/0009-0004-0133-1191
Shuang WuFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Yuhang LeiFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Shuang LiangFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Saihao WangFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Haodong DaiFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Yan WangFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Lei ChenFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Ye ZhaoFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Mailin GanFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Shijun XiaoNational Key Laboratory for Swine Genetic Improvement and Germplasm innovation Technology, Jiangxi Agricultural University, Nanchang, China.
Guangbin ZhouFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.ORCID https://orcid.org/0000-0002-4493-8311
Li ZhuFarm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Copper is essential for reproductive function, yet its accumulation can lead to cytotoxicity and cuproptosis. However, the specific molecular mechanisms underlying granulosa cell cuproptosis and follicular atresia remain unclear. Particularly, the molecular pathway by which tRNA-derived fragments (tRFs), recognized as crucial epigenetic regulators, are involved in the regulation of granulosa cell cuproptosis requires further elucidation. In this study, we indicated that copper accumulation disrupted mitochondrial respiration and protein lipoylation, resulting in impaired mitochondrial TCA cycling and subsequent cellular metabolic imbalance. Furthermore, a direct correlation was identified between tRFs and copper homeostasis. Functional analysis demonstrated that tRF-Gly-M3, produced by angiopoietin (ANG) splicing, was significantly upregulated in granulosa cells cuproptosis, and impaired mitochondrial function to induce cuproptosis by silencing the expression of GLS mRNA. tRF-Gly-M3 in exosomes secreted by cuproptosis-induced granulosa cells was high expression, and these exosomes could be delivered into oocytes. tRF-Gly-M3 also impaired oocytes mitochondrial metabolic function, inhibited oocytes maturation, first polar body extrusion and parthenogenesis via silencing GLS mRNA. Overall, our findings indicated that tRFs from granulosa cells could be intercellularly delivered to oocytes, effectively regulating oocyte development.

Indexed as

CuproptosisGranulosa CellsOocytesRNA, TransferAnimalsCell CommunicationExosomesFemaleMitochondriaSwineRNA, Transfer

Identifiers

PMID42060579
PMCPMC13132174

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.