Tubulin polyglutamylases TTLL4C and TTLL6B are essential for maintaining cytoskeletal integrity in

Tubulin polyglutamylation is a post-translational modification that modulates microtubule interactions with associated proteins and motor proteins, thereby contributing to the regulation of microtubule dynamics. Although its roles are well established in higher eukaryotes, the functional significance of this modification in protozoan parasites remains poorly understood. In the present study, we characterize two polyglutamylases, TTLL4C and TTLL6B, in the parasite IMPORTANCE: Post-translational modifications of microtubules, collectively known as the tubulin code, are increasingly recognized as key determinants in the modulation of microtubule properties. Notably, these modifications have been implicated in the pathogenesis of several diseases, including specific forms of neurodegeneration and ciliopathies. A comprehensive understanding of this regulatory layer is therefore of considerable biological and medical significance. Moreover, the conservation of tubulin post-translational modifications across eukaryotic evolution underscores their fundamental cellular importance. In this study, the protozoan parasite