Evidence map›Paper›PMID 42059401›Full record

ArticleChemistry & biodiversity2026

Anxiolytic and Memory Protective Effects of Withanolide D Isolated From Acnistus arborescens in Adult Zebrafish.

Cléia Rocha de Sousa Feitosa, Joilna Alves Da Silva, Jéssica Bezerra Maciel, Ivana Carneiro Romão, Thais Rocha Cavalcante, Francisco Anderson Nascimento Barros, Nicole de Abreu Bandeira, Emmanuel Silva Marinho, Jane Eire Silva Alencar De Menezes, Maria Kueirislene Amâncio Ferreira and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Cléia Rocha de Sousa FeitosaGraduate Program in Biological Chemistry, Universidade Regional do Cariri, Crato, Brazil.
Joilna Alves Da SilvaGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Jéssica Bezerra MacielGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Ivana Carneiro RomãoGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Thais Rocha CavalcanteGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Francisco Anderson Nascimento BarrosChemistry Course, Science and Technology Center, State University of Ceará, Fortaleza, Brazil.
Nicole de Abreu BandeiraChemistry Course, Science and Technology Center, State University of Ceará, Fortaleza, Brazil.
Emmanuel Silva MarinhoGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Jane Eire Silva Alencar De MenezesGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Maria Kueirislene Amâncio FerreiraGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Antonio Wlisses Da SilvaGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Márcia Machado MarinhoGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Matheus Nunes Da RochaGraduate Program in Natural Sciences, Campus Do Itaperi, State University of Ceará, Fortaleza, Brazil.
Otília Deusdênia Loiola PessoaGraduate Program in Chemistry, Universidade Federal do Ceará, Fortaleza, Brazil.
Pedro Henrique Jatai BatistaGraduate Program in Chemistry, Universidade Federal do Ceará, Fortaleza, Brazil.
Andreia Ferreira De Castro GomesCentre of Molecular and Environmental Biology (CBMA)/Aquatic Research Network (ARNET) Associate Laboratory, Campus De Gualtar, University of Minho, Braga, Portugal.
Alexandre Magno Rodrigues TeixeiraGraduate Program in Biological Chemistry, Universidade Regional do Cariri, Crato, Brazil.
Hélcio Silva Dos SantosGraduate Program in Biological Chemistry, Universidade Regional do Cariri, Crato, Brazil.

Funding

CNPQ-PQ 309349/2025-7Conselho Nacional de Desenvolvimento Científico e Tecnológico 304817/2025-2Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico FPD-0213-00369.01.00/23Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico ITR-0214-00060.01.00/23Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico UNI-0210-00315.01.00/23PQ/CNPq 306008/2022-0PQ/CNPq 308178/2021-1
6 · The paper itself

Abstract

This study evaluated the anxiolytic and memory-protective effects of withanolide D in adult zebrafish (Danio rerio). The compound was administered at doses of 4, 20, and 40 mg/kg and showed no signs of toxicity after 96 h of observation at any of the doses tested. In the open field test, withanolide D reduced locomotor activity, indicating a sedative-like effect. Nevertheless, in the light/dark test, doses of 20 and 40 mg/kg produced an anxiolytic-like response, suggesting that anxiolytic and motor effects may partially overlap depending on dose. The anxiolytic effect observed at the minimum effective dose (20 mg/kg) was reversed by flumazenil (FMZ), supporting the involvement of a benzodiazepine-sensitive GABA

Indexed as

Anti-Anxiety AgentsMemoryWithanolidesZingiberaceaeAnimalsAvoidance LearningDose-Response Relationship, DrugMolecular Docking SimulationMolecular StructureReceptors, GABA-AStructure-Activity RelationshipZebrafishAnti-Anxiety AgentsReceptors, GABA-Awithanolide DWithanolidesAcnistus arborescensanxietyDanio reriolearningwithanolide D

Identifiers

PMID42059401
PMCPMC13130859

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.