ArticleMicrobiology spectrum2026
Development of a high-throughput growth assay for bacteria or yeasts using an agar-based insoluble carbon source immobilization method.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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0 citing papers in PubMed.
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Authors and funding
6 authors.
Funding
Abstract
Polysaccharide degradation by microorganisms is essential in driving global nutrient cycling, developing renewable fuels or chemicals, and promoting human gut health. However, complex polysaccharides are often insoluble, making it challenging to study degradation with soluble enzymes or to measure microbial growth. Several protocols exist to address this challenge, including 3D-printed biomass containment devices or agar capture systems to facilitate the study of insoluble carbohydrate degradation. While these methods are functional, they are constrained by variable substrate loading and time-consuming preparation. To address these shortcomings, a 3D-printed pipette was designed and tested as part of a newly developed agar immobilization method to quantitatively monitor the degradation of insoluble polysaccharides for a 96-well microtiter assay. The pipette and method were validated via two mechanisms. First, growth analyses of
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Registered trials
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