Evidence map›Paper›PMID 42058948›Full record

ArticleTransboundary and emerging diseases2026

First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.

Siqi Zhang, Haoyuan Ma, Jiawei Zhao, Kai Yu, Jingrui Hao, Hao Yu, Jianyou Jin, Shuoning Cao, Xinpeng Ji, Shujiang Xue and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Siqi ZhangLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0005-9903-9072
Haoyuan MaLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0000-0002-2896-6814
Jiawei ZhaoLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0009-8745-0453
Kai YuLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0003-2423-5461
Jingrui HaoLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0009-3377-6503
Hao YuLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0006-9066-4645
Jianyou JinLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0009-6765-1696
Shuoning CaoLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0004-5942-6281
Xinpeng JiLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0003-7284-1036
Shujiang XueLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0000-1251-6651
Qiang LiLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.
Zhiqiang XuLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.
Shengwei JiLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0000-0001-7380-4809
Chenghui LiLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.
Zheng SunLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.
Jialiang XieLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0009-0004-8661-6718
Rui DuLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0000-0002-9703-3575
Xu GaoLaboratory for Animal Molecular Virology, Department of Veterinary Medicine, College of Agricultural, Yanbian University, Yanji, 1330002, China, ybu.edu.cn.ORCID https://orcid.org/0000-0002-2082-1215

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bovine coronavirus (BCoV) is an important viral agent associated with neonatal calf diarrhea (NCD), winter dysentery, and bovine respiratory disease, contributing to economic losses in cattle production worldwide. To date, BCoV sequence data from Yanbian, Northeast China, are limited in public datasets. In this study, we report the first successful isolation of a BCoV strain (BCoV-YBYJ) from a diarrheic calf in Yanbian using MDBK cells and characterize its replication in vitro and describe exploratory colon and lung findings following high-dose oral exposure in a murine model. The isolate replicated efficiently in MDBK cells. In mice, viral RNA was detected in the colon and lung with mild-to-moderate histological changes under high-dose exposure (nonnatural host). Phylogenetic analysis based on partial ORF1a, S, and M sequences showed that BCoV-YBYJ clustered with contemporary Chinese isolates within the available reference set, supporting regional genetic relatedness based on partial loci. In parallel, three SYBR Green I-based RT-qPCR assays targeting BCoV, bovine rotavirus (BRV), and bovine parvovirus (BPV) were established and analytically validated. These assays demonstrated satisfactory analytical sensitivity, specificity, and reproducibility within the validated dynamic range, and BCoV RNA was detected in 70% (140/200) of targeted diarrheic calf specimens (

Indexed as

Cattle DiseasesCoronavirus, BovineCoronavirus InfectionsDiarrheaReal-Time Polymerase Chain ReactionAnimalsBenzothiazolesCattleChinaDiaminesMiceOrganic ChemicalsPhylogenyQuinolinesBenzothiazolesDiaminesOrganic ChemicalsQuinolinesSYBR Green Ibovine coronavirusdifferential diagnosisneonatal calf diarrhearegional surveillanceSYBR Green I RT-qPCR

Identifiers

PMID42058948
PMCPMC13121856

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.