Evidence map›Paper›PMID 42058897›Full record

ArticleiScience2026

Heart failure pleural fluid impairs endothelial barrier through miR 501-3p mediated ZO 1 remodeling.

Chieh-Yung Wang, Tung-Yu Tiong, Ying Chen, Yi-Hsuan Lin, Vijesh Kumar Yadav, Chih-Ying Changchien, Chih-Feng Chian, Chen-Liang Tsai

Abstract read
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In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chieh-Yung WangDepartment of Internal Medicine, Tri-Service General Hospital Penghu Branch, National Defense Medical University, Taipei, Taiwan.
Tung-Yu TiongDivision of Thoracic Surgery, Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Ying ChenGraduate Institute of Biology and Anatomy, collage of medicine, National Defense Medical University, Taipei, Taiwan.
Yi-Hsuan LinGraduate Institute of Biology and Anatomy, collage of medicine, National Defense Medical University, Taipei, Taiwan.
Vijesh Kumar YadavDivision of Thoracic Surgery, Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Chih-Ying ChangchienDivision of Rheumatology, Immunology and Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan.
Chih-Feng ChianDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan.
Chen-Liang TsaiDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heart failure (HF) is frequently accompanied by pleural effusion, yet the biological impact of HF-associated pleural fluid on vascular endothelial function remains unclear. Here, we show that HF pleural fluid impairs endothelial barrier integrity and angiogenic capacity in human umbilical vein endothelial cells. Functional assays revealed increased permeability, reduced migration, and altered tube formation following treatment with patient-derived pleural fluid. Mechanistically, HF pleural fluid increased reactive oxygen species production and inflammatory signaling while downregulating tight junction protein ZO-1. Small RNA profiling identified miR-501-3p as a key mediator of these effects. Gain- and loss-of-function experiments demonstrated that miR-501-3p directly regulates ZO-1 expression and contributes to barrier disruption. These findings establish a microRNA-dependent mechanism linking HF pleural fluid to endothelial dysfunction and suggest a potential molecular pathway contributing to vascular complications in HF.

Indexed as

biochemical mechanismbiological scienceshealth sciencesmolecular mechanism of gene regulation

Identifiers

PMID42058897
PMCPMC13122223

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.