Evidence map›Paper›PMID 42058175›Full record

ArticleFrontiers in cellular and infection microbiology2026

The clinical value of metagenomic next generation sequencing in the diagnosis of non-neutropenic invasive pulmonary aspergillosis.

Huanhuan Zhong, Chao Sun, Yajie Lu, Xiaomin Cai, Min Cao, Li Wang, Chunlai Feng, Mengyue Song, Wenkui Sun, Minhua Shi and 13 more

Abstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Huanhuan Zhong *Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Chao Sun *Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Yajie Lu *Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Xiaomin Cai *Department of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Min CaoDepartment of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Li WangDepartment of Respiratory and Critical Care Medicine, Jiangsu Second Chinese Medicine Hospital, Nanjing, China.
Chunlai FengDepartment of Respiratory and Critical Care Medicine, Changzhou First People's Hospital, Changzhou, China.
Mengyue SongDepartment of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Nanjing Medical University, Nanjing, China.
Wenkui SunDepartment of Respiratory and Critical Care Medicine, Jiangsu Province Hospital, Nanjing, China.
Minhua ShiDepartment of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Soochow University, Suzhou, China.
Yujian TaoDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yangzhou University, Yangzhou, China.
Jun ZhouDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Yangzhou University, Yangzhou, China.
Cheng ChenDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.
Xin LuDepartment of Respiratory and Critical Care Medicine, Nanjing Jiangning Hospital, Nanjing, China.
Yuanqin LiDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Yueyan NiDepartment of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Yuchen CaiDepartment of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Jinjin ZhongDepartment of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Yuanyuan LiDepartment of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Weiwei WuDifei Medical Technology (Nanjing) Co., Ltd., Nanjing, China.
Yi ShiDepartment of Respiratory and Critical Care Medicine, Nanjing Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Min WangDepartment of Respiratory and Critical Care Medicine, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China.
Xin SuDepartment of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: This study aims to explore the performance of metagenomic next generation sequencing (mNGS) in the diagnosis of non-neutropenic invasive pulmonary aspergillosis (IPA) and its clinical application value. Methods: This multi-center study enrolled 293 suspected IPA patients who conducted mNGS from October 2020 to February 2024. These cases were classified into IPA group and non-IPA group according to IPA diagnostic criteria. We analyzed the diagnostic value of mNGS by comparing with sputum culture, BALF culture, serum and BALF GM test. Results: A total of 118 IPA patients (4 proven/113 probable/1 possible diagnosis) were included in our study. The most common Conclusion: mNGS demonstrated significant diagnostic value for IPA, exhibiting significantly higher sensitivity compared to current conventional microbiological tests while maintaining equivalent specificity. The combination of BALF mNGS with GM performed great sensitivity and negative predictive value. BALF specimens seemed to be superior to blood and sputum samples. However, for patients unable to undergo bronchoscopy, sputum and blood mNGS were still superior to other methods.

Indexed as

AspergillusHigh-Throughput Nucleotide SequencingInvasive Pulmonary AspergillosisMetagenomicsAdultAgedBronchoalveolar Lavage FluidFemaleGalactoseHumansMaleMannansMiddle AgedSensitivity and SpecificitySputumgalactomannanGalactoseMannansdiagnostic valueinvasive pulmonary aspergillosismetagenomic next generation sequencingmicrobiological testnon-neutropenic

Identifiers

PMID42058175
PMCPMC13121371

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.