ArticleFrontiers in bioengineering and biotechnology2026
Unique DNA impacts on transient Expi293
Article in Frontiers in bioengineering and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Mammalian transient gene expression technology is an essential tool for biotherapeutics engineering and production. Various reported transient expression systems in human embryonic kidney (HEK) 293 or Chinese hamster ovary (CHO) cells display different expression patterns and production yields, yet the mechanisms to which these patterns and yields can be attributed remain unknown. Methods: To identify the determining factors, we have dissected the transient expression processes through a three-snapshot approach for four commercially available transient production systems: Expi293 Results: The data showed that in response to titrated amounts of transfected DNAs for both the surface-display fusion molecule and the cytosolic GFP, Expi293 Conclusion: These results together suggested that the efficiency in handling transfected DNAs for protein synthesis (i.e., the ability to make intracellularly up-taken plasmid DNA complexes competent for DNA transcription in the nucleus) is likely a key limiting factor for effective transient production.
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