ArticleFrontiers in bioengineering and biotechnology2026
A fluffy nanofiber scaffold-based microenvironment enables a simplified, biology-centric scale-up strategy for mesenchymal stem/stromal cell culture.
Article in Frontiers in bioengineering and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Scalable suspension culture technologies are essential for the large-scale manufacturing of mesenchymal stem/stromal cells (MSCs). However, conventional microcarrier-based systems often fail to achieve scalable efficiency because cell-microcarrier dynamics vary across scales, necessitating complex, scale-dependent agitation protocols. To overcome this limitation, we designed a scale-up-oriented suspension culture system employing fluffy, fibrillated nanofiber scaffolds composed of chitosan and chitin. These nanofibers were readily suspended under continuous, gentle agitation and trapped cells on their surfaces, while spontaneously forming fluffy cell-scaffold aggregates through scaffold agglomeration. The low-adhesive chitosan nanofibers acted as physical spacers that prevented aggregate coalescence, thereby maintaining a microenvironment favorable for proliferation. By defining cell-scaffold aggregate size as the key scaling parameter-a biology-centric approach-, we successfully achieved scale-up from 30 mL to 5 L under continuous gentle agitation, yielding comparable specific growth rates of (3.66 ± 0.28) × 10
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