Evidence map›Paper›PMID 42057339›Full record

ArticleJournal of immunology research2026

Aspergillus fumigatus Gliotoxin Inhibits LC3-Associated Phagocytosis in Macrophages in a Calcium-Dependent Manner.

Yuqing Sun, Fangyan Chen, Ling Zhang, Caopei Zheng, Yu Wang, Yulin Zhang, Li Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunology research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuqing SunDepartment of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China, ccmu.edu.cn.ORCID https://orcid.org/0009-0005-4699-7461
Fangyan ChenDepartment for Disinfection and Infection Control, Chinese PLA Center for Disease Control and Prevention, Beijing, China, chinacdc.cn.ORCID https://orcid.org/0000-0001-9545-1952
Ling ZhangDepartment of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China, ccmu.edu.cn.ORCID https://orcid.org/0000-0003-1233-3892
Caopei ZhengDepartment of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China, ccmu.edu.cn.ORCID https://orcid.org/0000-0002-3138-6722
Yu WangDepartment of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China, ccmu.edu.cn.ORCID https://orcid.org/0009-0008-2822-2488
Yulin ZhangDepartment of Respiratory and Critical Care Medicine, Beijing Youan Hospital, Capital Medical University, Beijing, China, ccmu.edu.cn.ORCID https://orcid.org/0000-0002-1214-594X
Li HanDepartment for Disinfection and Infection Control, Chinese PLA Center for Disease Control and Prevention, Beijing, China, chinacdc.cn.ORCID https://orcid.org/0000-0001-9013-8414

Funding

Beijing Research Ward Excellence Program, BRWEP BRWEP2024W042180100National Natural Science Foundation of China 81571542National Natural Science Foundation of China 82172293
6 · The paper itself

Abstract

As a pivotal secondary metabolite of Aspergillus fumigatus, gliotoxin (GT) has many toxicological effects on mammalian cells; however, its function on LC3-associated phagocytosis (LAP) induced by A. fumigatus in macrophages is poorly understood. Here, it was found that pretreatment of macrophages with GT can significantly attenuate the conversion of LC3-II. In parallel, the expression of Rubicon, the putative indicator of LAP in macrophages, was dampened in a similar trend. Loss of ability to produce GT made the conidia of gliPΔ mutant of A. fumigatus induce more LC3-II conversion in THP1 cells, which could be inhibited by exogenous GT. Comparative transcriptomic analysis showed that GT can promote the expression of MAPK10, and the calcium-release regulatory pathway was enriched between the differentially expressed genes. Further, GT promotes the release of ROS at a concentration of 50 ng/mL, and inhibition by GT on LC3-II production in macrophages during A. fumigatus infection could be restored by pretreatment with calcium inhibitors but not affected by the inhibitors for JNK and siRNA for MAKP10. Collectively, our data demonstrated that GT exerts an inhibitory effect on LC3-associated phagocytosis in macrophages via a calcium-dependent mechanism, and MAPK10 exists downstream of LAP.

Indexed as

AspergillosisAspergillus fumigatusCalciumGliotoxinMacrophagesMicrotubule-Associated ProteinsPhagocytosisAnimalsHumansReactive Oxygen SpeciesTHP-1 CellsCalciumGliotoxinMicrotubule-Associated ProteinsReactive Oxygen SpeciesAspergillus fumigatuscalciumgliotoxinLC3-associated phagocytosisMAPK10

Identifiers

PMID42057339
PMCPMC13128990

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.