Evidence map›Paper›PMID 42054213›Full record

ArticleSTAR protocols2026

Protocol for culture and maintenance of canine induced pluripotent stem cells.

Kohei Shishida, Masaya Tsukamoto, Toshiya Nishimura, Shingo Hatoya

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kohei ShishidaDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan.
Masaya TsukamotoDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan.
Toshiya NishimuraPremium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Suita, Osaka 565-0871, Japan. Electronic address: tnishimu@nips.ac.jp.
Shingo HatoyaDepartment of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan. Electronic address: hatoya@omu.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for a feeder-free culture system for canine induced pluripotent stem cells (ciPSCs) using a recently developed medium, termed AR medium, on extracellular matrix (ECM)-coated dishes. We describe steps for thawing frozen ciPSC stocks, medium exchange, passaging, and cryopreservation. This technique enables stable colony formation, high viability, and consistent proliferation of ciPSCs during long-term maintenance. It provides a reproducible platform for downstream applications of ciPSCs, such as cell differentiation, gene editing, and disease modeling. For complete details on the use and execution of this protocol, please refer to Nishimura et al.

Indexed as

Cell Culture TechniquesInduced Pluripotent Stem CellsAnimalsCell DifferentiationCryopreservationCulture MediaDogsCulture MediaCell BiologyDevelopmental biologyStem Cells

Identifiers

PMID42054213
PMCPMC13138038

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.