Evidence map›Paper›PMID 42053999›Full record

ArticleVeterinary medicine and science2026

Molecular Characterization of Eustrongylides sp. Infecting Channa punctata in Bangladesh.

Salman Shahriar Nibir, Anita Rani Dey, Tanvir Rahman, Julfat Tasnim Suchona, Siyam Hossain, Savayan Sadad Bushra, Jubaida Parveen Juthy, Sandhya Paul, Jarin Tasnim Tanwi, Tamanna Tabassum and 1 more

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Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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11 authors.

Salman Shahriar NibirDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Anita Rani DeyDepartment of Parasitology, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0001-5462-7982
Tanvir RahmanDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.ORCID https://orcid.org/0000-0002-3586-0938
Julfat Tasnim SuchonaDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Siyam HossainDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Savayan Sadad BushraDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Jubaida Parveen JuthyDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Sandhya PaulDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Jarin Tasnim TanwiDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Tamanna TabassumDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Md Ali Reza FarukDepartment of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Funding

Bangladesh Agricultural University Research System 2021/113/BAU
6 · The paper itself

Abstract

backgroundEustrongylides (nematode: Dioctophymatidae) are parasitic nematodes of freshwater fishes and fish-eating birds. It has zoonotic potential and poses a threat to human health, as infection can occur through the consumption of raw or undercooked fish.

objectivesThe present study was designed to characterize Eustrongylides sp. isolated from Channa punctata using the Internal Transcribed Spacer 2 (ITS2) regions.

methodsIn this study, a total of 137 medium-sized C. punctata (17.5 ± 2.5 cm) were collected from different locations of Jamalpur and Netrokona district, Bangladesh and isolated 44 nematodes. DNA was extracted from the parasites, amplified in the ITS2 region and sequenced.

resultsAll the nematodes were microscopically identified as belonging to the genus Eustrongylides based on morphological characteristics, including the presence of inner and outer circle labial papillae at the anterior end and the esophago-intestinal junction. Initially, the sequences were confirmed up to the lowest taxonomic level possible using Basic Local Alignment Search Tool (BLAST). For phylogenetic analysis, reference sequences of Eustrongylides sp. were aligned, and two single nucleotide polymorphisms (SNPs) were detected. The average sequence identity percentage (%) within the studied sequences ranged from 99.7% to 99.8%. A maximum-likelihood (ML) tree was constructed which showed that the studied sequences clustered within the Eustrongylides sp. lineage and grouped with reference sequences, supported by moderate bootstrap values (82%).

conclusionsClose genetic similarity was observed when compared with reference sequences of Eustrongylides sp. Further studies on the investigation of genetic pattern of Eustrongylides sp. throughout the country are essential to control this parasite.

Indexed as

Channa punctataFish DiseasesNematodaAnimalsBangladeshDNA, HelminthDNA, Ribosomal SpacerMolecular Sequence DataPhylogenySequence Analysis, DNADNA, HelminthDNA, Ribosomal SpacerChanna punctataEustrongylides spnucleotide polymorphismphylogeny

Identifiers

PMID42053999
PMCPMC13127553

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