Evidence map›Paper›PMID 42053983›Full record

ArticleVeterinary medicine and science2026

Diagnostic Investigation of Two Cases of Oslerus osleri Infection in Dogs: Microscopic Challenges and Molecular Characterisation.

Jeremie Korchia, Vishnu Manikantan, Ashley K McGrew, Christina M Jeffries, Rebecca L Makii, Kelly McCord, Sriveny Dangoudoubiyam

Abstract readCase Reports
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In one paragraph

Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jeremie KorchiaDepartment of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.ORCID https://orcid.org/0000-0002-9344-6639
Vishnu ManikantanDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.
Ashley K McGrewDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine & Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Christina M JeffriesDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine & Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Rebecca L MakiiDepartment of Microbiology, Immunology and Pathology, College of Veterinary Medicine & Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Kelly McCordRoyal Vista Veterinary Specialists, Windsor, Colorado, USA.
Sriveny DangoudoubiyamDepartment of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.

Funding

CTSA Postdoctoral T32 at University of Colorado DenverT32TR004366 · NCATS · UNIVERSITY OF COLORADO DENVER · PI Lisa Cicutto · 2023 to 2026
$1.6M
NCATS NIH HHS T32 TR004366
6 · The paper itself

Abstract

backgroundThe diagnosis of certain lungworm infections in dogs relies heavily on bronchoalveolar lavage (BAL) cytology or lesion histopathology, especially in situations in which faecal diagnostics and bloodwork may have limited value. Differentiating morphologically similar parasitic larvae on BAL cytology, such as Oslerus osleri or Filaroides hirthi, may be challenging, and molecular approaches are not yet widely available.

objectiveDescription of two cases of O. osleri infection in young dogs, including (1) representative examples of BAL cytology and lesion-specific histopathologic findings and (2) molecular characterisation, based on targeted gene amplification and sequencing, from cytologic and histopathologic samples of O. osleri.

methodsTwo cases of O. osleri infection were investigated and followed in detail. BAL slides and tracheobronchial biopsy samples were analysed: six primer pairs spanning the ribosomal RNA gene of O. osleri were used to amplify the specific regions of genomic DNA from stained BAL slides (Case 1) and from formalin-fixed paraffin-embedded (FFPE) tracheobronchial biopsy (Case 2). Amplicon sequences were compared to O. osleri nucleotide sequences available in GenBank.

resultsThe amplicon sequences obtained from both cases were 100% identical, indicating that the infections in both dogs were caused by the same organism. BLAST analysis against the GenBank nucleotide database showed a 100% match with O. osleri, thereby confirming the diagnosis.

conclusionsPCR testing for O. osleri is feasible on genomic DNA obtained from stained BAL smears and sections of FFPE tissue. Further testing of these primers on other lungworms, such as F. hirthi, is warranted.

Indexed as

Dog DiseasesMetastrongyloideaStrongylida InfectionsAnimalsBronchoalveolar Lavage FluidDogsFemaleMalePolymerase Chain ReactionSequence Analysis, DNABaermann techniquebronchoalveolar lavage cytologycaninelungwormPCRverminous pneumonia

Identifiers

PMID42053983
PMCPMC13127552

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