ArticleAnalytical and bioanalytical chemistry2026
µ-Raman and GC-MS to interrogate the heterogeneity of deuterium-label incorporation on the single cell vs. fatty acid isotopologue level.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Understanding microbial metabolic functions at both bulk biomolecular and single-cell biomass levels requires precise tracing of isotope incorporation. Despite the established nature of compound-specific and single-cell stable isotope probing (SIP) approaches, a systematic comparison remains lacking. This study presents a comparative analysis of two SIP methods, utilizing deuterium from labelled glucose and heavy water to track incorporation into microbial fatty acids through gas chromatography-mass spectrometry (FAME-GC-MS) and into single cells via stable isotope Raman microspectroscopy (SIRM). Both methods exhibited consistent trends in deuterium incorporation across bacterial samples of Sphingomonas koreensis grown on various (deuterated) substrates in the presence of differing concentrations of D
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