Evidence map›Paper›PMID 42053801›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2026

A combination of QTL mapping and genome‑wide association study revealed key genes for heat tolerance in maize.

Xining Jin, Hangdan Qu, Pingxi Wang, Huaisheng Zhang, Tiantian Li, Xiangyuan Wu, Shilin Chen, Yaqiu Zhao, Fei Wang, Rui Song and 2 more

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Xining JinState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.ORCID http://orcid.org/0000-0003-1379-3460
Hangdan QuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Pingxi WangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Huaisheng ZhangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Tiantian LiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Xiangyuan WuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Shilin ChenState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Yaqiu ZhaoState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Fei WangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Rui SongState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Agricultural University, Zhengzhou, 450046, China. songrui2011@126.com.
Zhiyuan FuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Agricultural University, Zhengzhou, 450046, China. fuzhiyuan2004@163.com.
Xiaoxiang ZhangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, School of Agriculture, Henan Institute of Science and Technology, Xinxiang, 453003, China. zhangxx2020@hist.edu.cn.

Funding

Joint Research on Agricultural Improved Seed of Henan Province 2022010204Key Research and Development Project of Henan Province 241111114300Key Scientific and Technological Research Project of Henan Province 252102111130
6 · The paper itself

Abstract

key messageIntegrated QTL GWAS identified three key genes, whose heat ind uction during maize germination was verified via RNA seq and qRT PCR analysis. Heat stress during germination critically impairs maize seedling establishment and yield stability, yet its genetic basis remains poorly understood. Here, 21 quantitative trait loci (QTLs) for heat tolerance were identified in a recombinant inbred line (RIL) population under heat stress (39 °C vs. 28 °C), including a major hypocotyl diameter QTL (qHDT2-1, LOD = 23.68). Concurrently, genome-wide association study (GWAS) in an association panel detected 213 significant SNPs associated with heat tolerance. Population-stable SNPs, pleiotropic SNPs and high-impact QTLs were integrated to identify candidate genes. Transcriptome analysis and GO enrichment prioritized 612 candidate genes, among which six heat-induced genes were selected. Subsequent validation confirmed Zm00001d005300, Zm00001d023524, and Zm00001d026563 as core regulators forming a coordinated heat-response network. We also found that heat stress had a relatively minor impact on germination rates, but significantly affects biomass accumulation and tissue morphology. This study identifies key genetic targets for breeding heat tolerant maize.

Indexed as

Genes, PlantQuantitative Trait LociThermotoleranceZea maysChromosome MappingGene Expression Regulation, PlantGenome-Wide Association StudyGerminationPhenotypePolymorphism, Single Nucleotide

Identifiers

PMID42053801

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