Evidence map›Paper›PMID 42053410›Full record

ArticleThe FEBS journal2026

Long-read sequencing-based atlas of tissue-specific expression of DNM1L transcript variants.

Feng Yan, Jafar S Jabbari, Naomi X L Ling, Anne M Kong, Jia Q Truong, Marnie L Maddock, Ayeshah A Rosdah, Christopher G Langendorf, Lifang Zhang, Jarmon G Lees and 10 more

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Article in The FEBS journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

20 authors.

Feng YanThe Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Jafar S JabbariThe Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Naomi X L LingSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Anne M KongSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Jia Q TruongSTEM College, Engineering and Health, RMIT University, Melbourne, Australia.ORCID https://orcid.org/0000-0002-7328-189X
Marnie L MaddockSchool of Medical, Indigenous and Health Sciences, Molecular Horizons, University of Wollongong, Australia.
Ayeshah A RosdahFaculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia.
Christopher G LangendorfSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Lifang ZhangSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Jarmon G LeesSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Sebastian Bass-StringerSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Danise Ann OndaSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Alice PébayDepartment of Medicine and Surgery, University of Melbourne, Parkville, Australia.
Mirella DottoriSchool of Medical, Indigenous and Health Sciences, Molecular Horizons, University of Wollongong, Australia.
Kim LohSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Jessica K HolienSt Vincent's Institute of Medical Research, Fitzroy, Australia.ORCID https://orcid.org/0000-0002-8735-2871
Sean LalCharles Perkins Centre, The University of Sydney, Australia.
Nadia M DavidsonThe Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Jonathan S OakhillSt Vincent's Institute of Medical Research, Fitzroy, Australia.
Shiang Y LimSt Vincent's Institute of Medical Research, Fitzroy, Australia.ORCID https://orcid.org/0000-0002-0442-3655

Funding

National Health and Medical Research Council GNT2016547
6 · The paper itself

Abstract

Dynamin-related protein 1 (Drp1), encoded by DNM1L, is essential for mitochondrial fission, but its functional roles remain unclear due to isoform-specific effects from alternative splicing. Short-read RNA sequencing fails to resolve full-length isoforms involving distant exons, limiting our understanding. Here, we applied targeted long-read sequencing to profile full-length DNM1L transcripts in human left ventricle and induced pluripotent stem cell-derived cardiomyocytes, recovering all annotated isoforms with conserved expression patterns and isoforms 1-4 being the most abundant. Functional assays revealed that isoform abundance does not predict enzymatic activity. Extending this to six different mouse tissues, we identified distinct, tissue-enriched expression profiles. Functional rescue in Drp1-knockout mouse embryonic fibroblasts showed isoform-dependent differences in mitochondrial fission. Isoforms lacking the A-insert robustly rescued mitochondrial fission, with maximal activity observed for variants also lacking the B-insert (e.g. isoform b), consistent with a modulatory role of exon 3 in Drp1 activity. Our cross-species atlas integrates long-read transcriptomics with functional validation, revealing how isoform diversity underpins tissue-specific mitochondrial dynamics and physiological roles of Drp1.

Indexed as

DynaminsMyocytes, CardiacAlternative SplicingAnimalsFibroblastsHeart VentriclesHumansInduced Pluripotent Stem CellsMiceMice, KnockoutMitochondrial DynamicsOrgan SpecificityProtein IsoformsSequence Analysis, RNADNM1L protein, humanDnm1l protein, mouseDynaminsProtein IsoformscardiomyocytesDNM1LDrp1isoformsleft ventriclemitochondrial dynamics

Identifiers

PMID42053410
PMCPMC13669652

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.