ArticleNano letters2026
Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.
Article in Nano letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Stoichiometrically Defined Antibody-DNA Conjugates for Quantitative Super-Resolution Imaging.Nano letters · 2026Article
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2 authors.
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Abstract
Single-molecule localization microscopy (SMLM) techniques offer nanometer-scale resolution by stochastically localizing individual fluorescent molecules. Among these, DNA-mediated point accumulation for imaging in nanoscale topography (DNA-PAINT) stands out for its high multiplexing capability and excellent localization accuracy. In this work, we introduce a fast method using left-handed DNA (L-DNA) probes that show a lower background in DNA-PAINT experiments. The use of L-DNA reduces the background originating from unspecific imager and antibody binding. When fluorogenic probes are employed, emission from free binders is quenched, opening access to volumetric DNA-PAINT. Additionally, like fluorogenic right-handed (R-DNA-)PAINT, fluorogenic L-DNA-PAINT allows for lower integration times and faster accumulation of localizations. The binding kinetics, brightness, and localization precision of the fluorogenic R- and L-DNA probes are indistinguishable. Furthermore, fluorogenic left- and right-handed probes do not show cross-reactivity, thus expanding the range of available sequences for reliable multiplexed imaging. Integrating L-DNA-PAINT with a fluorogenic probe design significantly expands the experimental versatility of DNA-PAINT.
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