Evidence map›Paper›PMID 42052934›Full record

ArticleMediators of inflammation2026

Identification of Anti-Inflammatory and Analgesic Mechanisms of Naru-3 Wei Pill Using Animal Models, UHPLC-QE-MS, and Integrated Network Pharmacology.

Zhiqiang Han, Husile Kang, Aidi Zhang, Qigeqin, Baolechaolu, Wulanqiqige, Huan Wang, Yanhua Xu, HaSi, Anda and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhiqiang HanInstitute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No.1742, Huolinhe Street, Horqin Area, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0000-0003-4643-0631
Husile KangDepartment of Medical Education, Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0009-0007-6414-6104
Aidi ZhangClinical Medical College, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China, imun.edu.cn.ORCID https://orcid.org/0009-0002-0857-3287
QigeqinClinical Medical College, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China, imun.edu.cn.
BaolechaoluMedical Innovation Center for Nationalities, Inner Mongolia Medical University, Huhhot, 010110, Inner Mongolia, China, immu.edu.cn.ORCID https://orcid.org/0009-0003-2907-875X
WulanqiqigeCollege of Mongolian Medicine, Inner Mongolia Medical University, Huhhot, 010110, Inner Mongolia, China, immu.edu.cn.ORCID https://orcid.org/0009-0008-6354-0385
Huan WangClinical Medical College, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China, imun.edu.cn.ORCID https://orcid.org/0000-0002-5457-665X
Yanhua XuInstitute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No.1742, Huolinhe Street, Horqin Area, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0000-0001-5396-942X
HaSiInstitute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No.1742, Huolinhe Street, Horqin Area, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0000-0002-1927-1260
AndaInstitute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No.1742, Huolinhe Street, Horqin Area, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0000-0002-7912-0587
Lan XueInstitute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia Minzu University, No.1742, Huolinhe Street, Horqin Area, Tongliao, 028000, Inner Mongolia, China, nmgmzdxfsyy.com.ORCID https://orcid.org/0009-0006-4224-3360

Funding

Science and Technology Program of Inner Mongolia Autonomous Region 2022YFSH0114
6 · The paper itself

Abstract

backgroundNaru-3 Wei Pill is a traditional Mongolian Medicine (TMM) that has anti-inflammatory, analgesic, and antibacterial effects. The present study aimed to demonstrate the anti-inflammatory and analgesic effect of Naru-3 Wei Pill, identify the core components and key targets and determine the pharmacological basis and mechanism of its blood-borne components.

methodsThe anti-inflammatory and analgesic effects of Naru-3 pills were studied using hot plate, tail flick, and acetic acid-induced writhing tests. The blood-borne components of Naru-3 Wei Pill were identified using ultrahigh-performance liquid chromatography with Q-Exactive mass spectrometry (UHPLC-QE-MS), and their targets were screened using ChEMBL, TCMIO, GeneCards, and TTD databases. Network construction, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and protein-protein interaction (PPI) network was performed. Molecular dynamics methods, PCR and western blot, were used for verification of the results.

resultsMultiple pain models results indicate that Naru-3 pills exert anti-inflammatory and analgesic effects by modulating the PI3K signaling pathways and reducing inflammation factors (interleukin [IL]-6). A total of 35 blood-borne components were identified, acting on 291 targets involving 172 pathways. The core targets included AKT1, SRC, mitogen-activated protein kinase 14 (MAPK14), and ESR1. Molecular docking and dynamics simulations conformed strong binding affinity of the complexes formed between Rhein and AKT1, MAPK1, and SRC, and between genistein and SRC. PCR and western blot confirm the regulation of AKT/PI3K pathway of Naru-3 pills in animal models.

conclusionNaru-3 pills significantly prolonged pain thresholds and reduced pain behaviors in mice. The study identified the material basis and mechanisms of its anti-inflammatory and analgesic effects, providing a foundation for further research.

Indexed as

AnalgesicsAnti-Inflammatory AgentsDrugs, Chinese HerbalNetwork PharmacologyAnimalsChromatography, High Pressure LiquidMaleMass SpectrometryMiceMolecular Docking SimulationPainPhosphatidylinositol 3-KinasesSignal TransductionAnalgesicsAnti-Inflammatory AgentsDrugs, Chinese HerbalPhosphatidylinositol 3-Kinasesanalgesicanti-inflammatoryblood-borne componentsmolecular dynamicsNaru-3 Wei Pillnetwork pharmacology

Identifiers

PMID42052934
PMCPMC13126247

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.