ArticlePeerJ2026
Lateral flow-based assay for simple and rapid visual detection of Asian-type DEL.
Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Background: Where a high prevalence of Asian-type DEL ( Method: In this study, we simplified the performance of the polymerase chain reaction (PCR) combined with a lateral flow assay procedure (PCR-LF), providing a rapid and more sensitive detection of the PCR product and validated it for the identification of Asian-type DEL in samples with a serologically D- phenotype. Result: In contrast to conventional PCR with agarose gel electrophoresis (PCR-AGE), any PCR products were detected by the lateral flow assay within 10 minutes. This assay accelerates the PCR work schedule and reduces post-PCR detection steps. PCR-LF, PCR-AGE, and Sanger-sequencing assays showed concordant results. The developed PCR-LF assay was more sensitive than an existing PCR-AGE assay for the Conclusion: PCR-LF was developed to detect Asian-type DEL. This method being rapid and easy to perform would be useful for identifying the Asian-type DEL which serological methods are unable to detect. This could improve blood transfusion management and for the selection of the right blood products to prevent anti-D alloimmunization.
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