Evidence map›Paper›PMID 42051309›Full record

ArticlebioRxiv : the preprint server for biology2026

A chorionic gonadotropin assay enables non-invasive detection of ovulation and early pregnancy in a New World primate model.

Keiko Kishimoto, Takuma Soga, Akio Iio, Masahiko Hatakeyama, Satoru Kawai, Michiko Kamioka, Jinsho Aoki, Yuka Bunzui, Yuko Yamada, Miho Kohara and 11 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Keiko KishimotoDivision of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.
Takuma SogaCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Akio IioCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Masahiko HatakeyamaCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Satoru KawaiAmami Laboratory of Medical Science, The Institute of Medical Science, The University of Tokyo, 802 Tean, Setouchi-cho, Oshima-gun, Kagoshima, 894-1531, Japan.
Michiko KamiokaDivision of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.
Jinsho AokiCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Yuka BunzuiCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Yuko YamadaDivision of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.
Miho KoharaTsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, 1-1 Hachimandai, Tsukuba-shi, Ibaraki, 305-0843, Japan.
Yoko KurotakiDepartment of Development Research Translational Research Division, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.
Wakako KumitaDivision of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.
Julie Brent-CumminsNational Institutes of Health (NIH), National Institute of Neurological Disorders and Stroke, 9000 Rockville Pike, 49 Convent Dr., Bethesda, MD 20882, United States of America.
Sang Su OhNational Institutes of Health (NIH), National Institute of Neurological Disorders and Stroke, 9000 Rockville Pike, 49 Convent Dr., Bethesda, MD 20882, United States of America.
Milton HerreraNational Institutes of Health (NIH), National Institute of Neurological Disorders and Stroke, 9000 Rockville Pike, 49 Convent Dr., Bethesda, MD 20882, United States of America.
Lian BikNational Institutes of Health (NIH), National Institute of Neurological Disorders and Stroke, 9000 Rockville Pike, 49 Convent Dr., Bethesda, MD 20882, United States of America.
Heather NarverNational Institutes of Health (NIH), National Institute on Alcohol Abuse and Alcoholism, 5625 Fishers Lane, Rockville, MD 20852, United States of America.
Tadashi SankaiTsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, 1-1 Hachimandai, Tsukuba-shi, Ibaraki, 305-0843, Japan.
Tomoji MashimoAmami Laboratory of Medical Science, The Institute of Medical Science, The University of Tokyo, 802 Tean, Setouchi-cho, Oshima-gun, Kagoshima, 894-1531, Japan.
Kazumasa FukasawaCLEA Japan, Inc., 1-2-7 Higashiyama, Meguro-ku, Tokyo, 153-8533, Japan.
Erika SasakiDivision of Advanced Physiology, Central Institute for Experimental Medicine and Life Science (CIEM), 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 216-0821, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early detection of ovulation and pregnancy in the common marmoset is crucial for reproductive studies, yet hCG kits lack cross-reactivity with marmoset CG, and current methods remain labor-intensive. Here, we developed monoclonal antibodies against marmoset CGα and CGβ, and established a non-invasive immunochromatographic CG assay. By eliminating invasive blood sampling, this assay supports 3Rs principles and enables practical endocrine monitoring. The assay detected urinary CG surges preceding ovulation, enabling efficient embryo recovery through artificial insemination (75%). Early pregnancy was detected at approximately 17 days post-ovulation. In addition, pregnancy detection in squirrel monkeys suggests conservation of CG features among certain New World primates. Overall, this simple, non-invasive assay provides a practical tool for marmoset research and establishes a foundation for future conservation-oriented reproductive monitoring following appropriate species-specific validation.

Indexed as

chorionic gonadotropincommon marmosetearly pregnancy and ovulation detectionNew World monkeys

Identifiers

PMID42051309
PMCPMC13119320

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.