Evidence map›Paper›PMID 42050644›Full record

ArticleNeuropsychopharmacology reports2026

Transcranial Calcium Macro-Imaging From the Auditory Cortex of Thy1-Cre-Driven GCaMP8 Transgenic Rats.

Manavu Tohmi, Hisaaki Namba, Meiko Kawamura, Ryuichi Hishida, Shota Iguchi, Kazuto Kobayashi, Natsuki Matsushita, Tomoji Mashimo, Rie Natsume, Kenji Sakimura and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropsychopharmacology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Manavu TohmiDepartment of Physiological Sciences, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama, Japan.
Hisaaki NambaDepartment of Physiological Sciences, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama, Japan.
Meiko KawamuraDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.
Ryuichi HishidaDepartment of Neuroscience of Disease, Brain Research Institute, Niigata University, Niigata, Japan.ORCID https://orcid.org/0000-0002-7856-4127
Shota IguchiDepartment of Physiological Sciences, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama, Japan.
Kazuto KobayashiDepartment of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan.
Natsuki MatsushitaDivision of Laboratory Animal Research, Aichi Medical University School of Medicine, Aichi, Japan.
Tomoji MashimoDivision of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, the University of Tokyo, Tokyo, Japan.ORCID https://orcid.org/0000-0001-7543-7301
Rie NatsumeDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.
Kenji SakimuraDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.ORCID https://orcid.org/0000-0002-8091-8879
Hiroyuki NawaDepartment of Physiological Sciences, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama, Japan.

Funding

Japan Society for the Promotion of Science 18K06518Japan Society for the Promotion of Science 21K18242Japan Society for the Promotion of Science 22H04922
6 · The paper itself

Abstract

backgroundGenetically encoded calcium indicators such as GCaMP enable us to monitor neuronal activities from living animals at synaptic levels as well as at whole-brain levels. However, the genetic variation of GCaMP transgenic rats is limited compared with the various demands for neuroscience studies on rats.

methodWe established two transgenic rat lines: one expressing Cre recombinase under the control of the Thy1 promoter and another knock-in line carrying a floxed STOP cassette followed by GCaMP8 inserted into the β-actin locus. We mated these two transgenic rats and produced the Thy1-Cre-driven GCaMP8-expressing rats.

resultsApproximately 80% of cortical neurons were positive for the GCaMP immunoreactivity. The GCaMP8-expressing rats were subjected to transcranial calcium imaging from the auditory cortex under anesthesia. With this simple procedure, we were able to detect the maximum responses to 68 dB click sounds;ΔF/F

conclusionsThese results suggest that the present GCaMP8-expressing rats as well as their parental transgenic rat strains offer a new tool for cortical macro-imaging and functional mapping with the transcranial approach.

Indexed as

Auditory CortexCalciumAcoustic StimulationAnimalsFemaleIntegrasesMaleNeuronsRatsRats, TransgenicThy-1 AntigensCalciumCre recombinaseIntegrasesThy-1 Antigensauditory cortexfunctional mappingGCaMPThy1transgenic rat

Identifiers

PMID42050644
PMCPMC13124658

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.