Evidence map›Paper›PMID 42050226›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2026

Integrated metabolomic profiling reveals metabolomic responses by epicardial and myocardial stromal cells to ischemia.

Dongwei Sun, Alex Postajian, Edgmin Rostomian, Yu Chen, Junyoung O Park, Vedi Hatamian, Kevin Babakhan Vartanian, Finosh G Thankam

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Article in Metabolomics : Official journal of the Metabolomic Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Dongwei SunSmidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.ORCID http://orcid.org/0000-0002-8316-5745
Alex PostajianDepartment of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
Edgmin RostomianDepartment of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
Yu ChenMolecular Instrumentation Center, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
Junyoung O ParkDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0001-9869-8993
Vedi HatamianDepartment of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
Kevin Babakhan VartanianDepartment of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
Finosh G ThankamDepartment of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA. FThankam@westernu.edu.ORCID http://orcid.org/0000-0001-7285-9808

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCardiac ischemia induces substantial metabolomic reprogramming, which dysregulates cardiomyocytes (CMs) and non-myocyte stromal cell populations. The stromal cells derived from epicardial adipose tissue (EAT) and ventricle are critical for extracellular matrix (ECM) remodeling, paracrine signaling, and myocardial homeostasis. However, the metabolomic content and responses of EAT-derived stromal cells (EATDS) and ventricular stromal cells (VSCs) remain unknown. METHODOLOGY: This study employed untargeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics to characterize ischemia-driven metabolic reprogramming in EATDS and VSCs harvested from swine hearts. Ischemia was simulated using the standard ischemic buffer (pH 6.2) for 2 h.

resultsMetabolomic screening revealed 65 and 68 metabolites, respectively, for EATDS and VSCs. Results revealed extensive downregulation of amino acid biosynthesis, redox pathways, and mitochondrial metabolism, alongside selective upregulation of glycolytic and cofactor-associated metabolites. Pathway enrichment analyses indicated significant suppression of the TCA cycle, one-carbon metabolism, glutathione cycling, and branched-chain amino acid degradation, reflecting impaired bioenergetic and antioxidant capacity. Adaptive responses included the enrichment of glycolysis, β-alanine, and glyoxylate/dicarboxylate metabolism, consistent with metabolic plasticity under hypoxic conditions. Network-based analyses linked these metabolic shifts to inflammatory pathways. Functional assays demonstrated that sarcosine, pyroglutamic acid, and 3-hydroxypropionic acid modulate the gene expression of cardiac regenerative biomarkers, including GATA4, Nkx2.5, TROP-I, LGALS1, TBX5, and IRX4.

conclusionsThese findings suggest that ischemia-induced metabolomic changes exert transcriptional control over cardiac remodeling programs, emphasizing the regulatory potential of metabolite-gene interactions. Such an integrated metabolomic transcriptional response highlights novel therapeutic targets for modulating cellular resilience and heart regeneration following ischemic heart disease.

Indexed as

MetabolomicsMyocardial IschemiaMyocardiumPericardiumStromal CellsAnimalsChromatography, LiquidEpicardial Adipose TissueMetabolic ReprogrammingMetabolomeMyocytes, CardiacSwineCardiac homeostasisEpicardial adipose stromal cellsMetabolic reprogrammingMyocardial ischemiaVentricular stromal cells

Identifiers

PMID42050226
PMCPMC13124829

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.