ArticleCell2026
Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.
Abstract read
In one paragraph
Article in Cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- A novel ILP framework to identify compensatory pathways in genetic interaction networks with GIDEON.Bioinformatics (Oxford, England) · 2026Article
- Human genetics across levels of biological organization.Nature reviews. Genetics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
90 authors.
Maximilian BillmannDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA; Institute of Human Genetics, School of Medicine and University Hospital Bonn, University of Bonn, Bonn, Germany.
Michael CostanzoDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Xiang ZhangDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Arshia Z HassanDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Mahfuzur RahmanDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Kevin R BrownProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Katherine S ChanProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Amy Hin Yan TongDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Carles PonsInstitute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute for Science and Technology, Barcelona, Spain.
Henry N WardDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA; Graduate Program in Bioinformatics and Computational Biology (BICB), University of Minnesota, Minneapolis, MN, USA.
Catherine RossDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Jolanda van LeeuwenDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Michael AreggerDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Keith A LawsonDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Barbara MairDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Amy F RothDepartment of Pharmacology, Wayne State University School of Medicine, Detroit, MI, USA.
Nesli E SenDepartment of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland.
Duncan T ForsterDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Guihong TanDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Patricia MeroProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Sanna N MasudDonnelly Centre, University of Toronto, Toronto, ON, Canada; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Yoonkyu LeeDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA; Graduate Program in Bioinformatics and Computational Biology (BICB), University of Minnesota, Minneapolis, MN, USA.
Magali Aguilera-UribeProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Matej UšajDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Sylvia M T AlmeidaDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Kamaldeep AulakhProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Urvi BhojooDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Saba BirkadzeProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Nathaniel BudijonoDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Xunhui CaiInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Joseph J CaumannsDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Jordan J ChalmersProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Megha ChandrashekharDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Daniel ChangDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Ryan ClimieDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Kuheli DasguptaProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Adrian DrazicDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Biomedicine, University of Bergen, Bergen, Norway.
Jose I Rojas EcheniqueDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Rafael GacesaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Adrian Granda FariasProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Andrea HabsidProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Ira HoreckaDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Kristin KantautasDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Fenghu JiInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Dae-Kyum KimDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Surgery, McGill University, Cancer Research Program, Research Institute of McGill University Health Centre, Montreal, QC, Canada.
Seon Yong LeeProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Wendy LiangDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Hyobin Julianne LimDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Kevin LinDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA; Graduate Program in Bioinformatics and Computational Biology (BICB), University of Minnesota, Minneapolis, MN, USA.
Xueyibing LuDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Michael MaierGenome Institute of Singapore (GIS), Agency for Science, Technology and Research (A(∗)STAR), Singapore, Singapore.
Babak NamiProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Allison NixonDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Nicholas MikolajewiczProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Milad MokhtaridoostProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
Lyudmila NedyalkovaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Thomas RohdeInstitute of Human Genetics, School of Medicine and University Hospital Bonn, University of Bonn, Bonn, Germany.
Maria Sartori RodriguesDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Martin SosteDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Eric SchultzDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Wen WangDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA.
Ashwin SeetharamanDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Ermira ShuteriqiDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Olga SizovaProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada.
David Thomson TaylorProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Institute for Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Maria TereshchenkoDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
David TieuDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Jacob TurowecDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Tajinder UbhiDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Biochemistry, University of Toronto, Toronto, ON, Canada.
Sylvia VarlandDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Biomedicine, University of Bergen, Bergen, Norway.
Kyle E WangDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Zi Yang WangDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Jiarun WeiProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Yu-Xi XiaoProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Philipp G MaassProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Bruno ReversadeGenome Institute of Singapore (GIS), Agency for Science, Technology and Research (A(∗)STAR), Singapore, Singapore.
Grant W BrownDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Biochemistry, University of Toronto, Toronto, ON, Canada.
Benjamin F CravattDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA, USA.
Scott J DixonDepartment of Biology, Stanford University, Stanford, CA, USA.
Haley D M WyattDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
Hannes L RöstDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Frederick P RothDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Tian XiaSchool of Software Engineering, Huazhong University of Science and Technology, Wuhan, China.
Gary D BaderDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Robbie LoewithDepartment of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland.
Nicholas G DavisDepartment of Pharmacology, Wayne State University School of Medicine, Detroit, MI, USA.
Brenda AndrewsDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. Electronic address: brenda.andrews@utoronto.ca.
Chad L MyersDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, USA; Graduate Program in Bioinformatics and Computational Biology (BICB), University of Minnesota, Minneapolis, MN, USA. Electronic address: chadm@umn.edu.
Jason MoffatProgram in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; Institute for Biomedical Engineering, University of Toronto, Toronto, ON, Canada. Electronic address: jason.moffat@sickkids.ca.
Charles BooneDonnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan; Canadian Institute for Advanced Research (CIFAR), Toronto, ON, Canada. Electronic address: charlie.boone@utoronto.ca.
Funding
Mapping the reference genetic network of a eukaryotic cellR01HG005853 · NHGRI · UNIVERSITY OF TORONTO · PI ANDREWS, BRENDA JEAN, BOONE, CHARLES · 2010 to 2025
$7.6MNHGRI NIH HHS R01 HG005853
Abstract
Deciphering how genes interact within human cells is essential for understanding their functional wiring and for developing targeted therapeutic strategies. In this study, we present a genome-scale map of genetic interactions in the human haploid cell line HAP1, based on CRISPR-based perturbation of ∼4 million gene pairs. The resulting network comprises ∼89,000 high-confidence gene-gene interactions, organizing genes into hierarchical modules corresponding to protein complexes and pathways, biological processes, and cellular compartments, mirroring principles observed in yeast and highlighting the functional architecture of a human cell. This large-scale genetic network complements the DepMap gene co-essentiality network by capturing unique functional information, uncovering roles of previously uncharacterized genes, and identifying molecular determinants of cancer-cell-line-specific genetic dependencies. This study presents a general data-driven strategy for systematically exploring the roles of genes and their functional connections in human cell lines.
Indexed as
Gene Regulatory NetworksGenes, EssentialCell LineEpistasis, GeneticGenome, HumanHaploidyHumansSaccharomyces cerevisiaegenetic interactionsgenetic network conservationgenetic suppressiongenome-scale genetic interaction networkgenome-wide CRISPR screenshuman haploid cellssynthetic lethality
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.