Evidence map›Paper›PMID 42048549›Full record

ArticleG3 (Bethesda, Md.)2026

Image-based high-throughput phenotyping enables genetic analyses of pod morphological traits in mungbean (Vigna radiata (L.) R. Wilczek).

Venkata Naresh Boddepalli, Talukder Zaki Jubery, Steven B Cannon, Somak Dutta, Baskar Ganapathysubramanian, Arti Singh

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Venkata Naresh BoddepalliDepartment of Agronomy, Iowa State University, Ames, IA 50011, United States.ORCID 0009-0008-0216-9156
Talukder Zaki JuberyDepartment of Mechanical Engineering, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0003-4107-1617
Steven B CannonDepartment of Agronomy, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0003-2777-8034
Somak DuttaDepartment of Statistics, Iowa State University, Ames, IA 50011, United States.ORCID 0000-0002-5613-8987
Baskar GanapathysubramanianDepartment of Mechanical Engineering, Iowa State University, Ames, IA 50011, United States.
Arti SinghDepartment of Agronomy, Iowa State University, Ames, IA 50011, United States.

Funding

AI Institute for Resilient Agriculture (USDA-NIFA) #2021-67021-35329AI Institute for Resilient Agriculture (USDA-NIFA) #2026-67013-45812NSF-funded COntext Aware LEarning for Sustainable CybEr-Agricultural Systems #2021-1954556United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) #2022-67013-37120USDA-Agricultural Research Service (USDA-ARS) 5030-21000-071-000D (SBC)USDA NIFA #2023-70412-41087U.S. Department of Agriculture
6 · The paper itself

Abstract

Mungbean (Vigna radiata (L.) R. Wilczek) is a vital source of digestible proteins and is well-suited for the plant-based protein industry. In this study, we analyzed pod morphological traits in the Iowa Mungbean Diversity (IMD) panel of 372 genotypes (2022-2023) using image-analysis-based phenotyping on 2,418 pod images. Pod morphological traits were extracted using deep learning image analysis, achieving excellent agreement with manual measurements (r > 0.96 for pod length (PL) and seed-per-pod (SPP)). Four complementary genome-wide association studies models identified 65 significant SNPs (-log10(P) ≥ 5.56) associated with pod curvature, length, width, and SPP traits. A significant SNP (5_35265704) on chromosome 4 was linked to pod dimensional traits, length, width, and curvature. A candidate gene, Virad04G0076900, located 15.6 kb from this SNP, is part of the GH3 gene family and has an Arabidopsis ortholog (AT4G27260) known for influencing organ elongation, pod, and seed development. Another SNP, 5_210437 on chromosome 6, has been found to be significantly associated with both PL and SPP. A candidate gene, Virad06G0002400 (36.5 kb from this SNP), encodes a potassium transporter and shares homology with the Arabidopsis gene HAK5 (AT4G13420), known to influence pod growth. Image-based measurements achieved genomic prediction accuracies ranging from 0.61 to 0.85 across various traits, demonstrating comparable accuracy to manual methods for linear traits and up to 22% improvement for complex shape traits. These results highlight the potential of deep learning-assisted phenomics integrated with genomic tools to accelerate selection for improved pod architecture in mungbean breeding programs across the Midwestern United States and globally.

Indexed as

PhenotypeQuantitative Trait, HeritableVignaChromosome MappingGenome-Wide Association StudyGenotypeImage Processing, Computer-AssistedPolymorphism, Single NucleotideQuantitative Trait LociSeedsand high-throughput phenotypinggenomic predictionGWASimage analysismungbean

Identifiers

PMID42048549
PMCPMC13233092

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.