Evidence map›Paper›PMID 42047888›Full record

ArticleJournal of computer-aided molecular design2026

Design, purification, and biophysical characterization of a multi-epitope vaccine construct made from outer membrane proteins of Pseudomonas aeruginosa.

Yogeshwar Devarakonda, Syed Mohammad Omer Ali, Juan Edwin James, Abhinav Cheruvu Manikantha Sai, Sakshi Batra, Kirtimaan Syal

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Article in Journal of computer-aided molecular design, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Yogeshwar DevarakondaGenetics and Molecular Microbiology Laboratory, Department. of Biological Sciences, Institute of Eminence, Birla Institute of Technology and Sciences-Pilani, Hyderabad campus, Shamirpet-Mandal, Hyderabad, Telangana, 500078, India.
Syed Mohammad Omer Ali *Genetics and Molecular Microbiology Laboratory, Department. of Biological Sciences, Institute of Eminence, Birla Institute of Technology and Sciences-Pilani, Hyderabad campus, Shamirpet-Mandal, Hyderabad, Telangana, 500078, India.
Juan Edwin James *Indian Institute of Science Education and Research, Mohali, Chandigarh, India.
Abhinav Cheruvu Manikantha SaiGenetics and Molecular Microbiology Laboratory, Department. of Biological Sciences, Institute of Eminence, Birla Institute of Technology and Sciences-Pilani, Hyderabad campus, Shamirpet-Mandal, Hyderabad, Telangana, 500078, India.
Sakshi BatraMalla Reddy Institute of Medical Sciences, Hyderabad, India.
Kirtimaan SyalGenetics and Molecular Microbiology Laboratory, Department. of Biological Sciences, Institute of Eminence, Birla Institute of Technology and Sciences-Pilani, Hyderabad campus, Shamirpet-Mandal, Hyderabad, Telangana, 500078, India. ksyal@hyderabad.bits-pilani.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pseudomonas aeruginosa is increasingly becoming resistant to multiple drugs and is held responsible for high rate of mortality and morbidity across the globe. This study aims to examine proteins from outer membrane such as OprE, OprF, OprC, and OprG, for the multi-epitope vaccine design. In this article, the prediction of epitopes for helper T-cells, cytotoxic T-cells, and B-cell epitopes was carried out by the application of various immune-informatics tools. All the predicted epitopes were aligned and assembled into a peptide sequence along with linkers and adjuvant sequences. Further, secondary structure and three-dimensional structure were predicted for the multiepitope construct. The vaccine construct designs were evaluated and validated for allergenicity, toxicity, and antigenicity. The validation of the predicted structure was carried out by determining its physiochemical properties by the application of ProtParam. Protein docking and molecular-dynamic simulation confirmed strong and stable interaction between the vaccine construct and Toll-like receptor-4. The vaccine construct was cloned into pET-29b vector and expressed in Escherichia coli. The designed multiepitope vaccine construct was overexpressed and purified by the application of Ni-NTA affinity chromatography and subjected to SDS-PAGE analysis. The circular dichroism spectroscopy analysis revealed it to be stable and structured. The hemolysis assay demonstrated minimal RBC toxicity suggesting that it was safe to use. The designed vaccine construct could activate both humoral and cellular immune responses as demonstrated by the advanced immunoinformatic approach making it a promising vaccine construct for protection against P. aeruginosa.

Indexed as

Bacterial Outer Membrane ProteinsEpitopesPseudomonas aeruginosaPseudomonas VaccinesAnimalsEpitopes, B-LymphocyteEpitopes, T-LymphocyteHumansImmunoinformaticsMolecular Docking SimulationMolecular Dynamics SimulationProtein Subunit VaccinesToll-Like Receptor 4Bacterial Outer Membrane ProteinsEpitopesEpitopes, B-LymphocyteEpitopes, T-LymphocyteProtein Subunit VaccinesPseudomonas VaccinesToll-Like Receptor 4Antimicrobial resistanceMultiepitope vaccine designOuter membrane proteins (OprC, OprE, OprF, OprG)Pseudomonas aeruginosaReversevaccinology

Identifiers

PMID42047888

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.