Evidence map›Paper›PMID 42047747›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Connexin 43-Enriched Vesicles Improve Synchronization in hiPSC-Derived Cardiomyocytes.

Nima Momtahan, Anna K McClain, Andrea Trementozzi, Sogu Sohn, Lingxia Qiao, Chi Zhao, Padmini Rangamani, Jeanne C Stachowiak, Janet Zoldan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nima MomtahanDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.ORCID https://orcid.org/0000-0002-4220-2351
Anna K McClainDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Andrea TrementozziDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Sogu SohnDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Lingxia QiaoDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Chi ZhaoDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Padmini RangamaniDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Jeanne C StachowiakDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Janet ZoldanDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, USA.ORCID https://orcid.org/0000-0001-7089-7683

Funding

Protein Networks as Synergistic Drivers of Membrane RemodelingR35GM139531 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Jeanne Casstevens Stachowiak · 2021 to 2026
$4.0M
Army Research Office W911NF2310249National Science Foundation 2002652National Science Foundation 2228270National Science Foundation 2530428NIH HHS R35GM139531Texas Biologics TXB-24-04Welch Foundation F-2257
6 · The paper itself

Abstract

Human induced pluripotent stem cell-derived cardiomyocytes are valuable for studying cell-cell communication and synchronization, but remain immature and often lack robust electrical and mechanical coupling. To address this, we investigated gap junction-mediated communication and developed plasma membrane vesicles enriched in functional connexin hemichannels, termed Connectosomes, to enhance intercellular coupling. Connectosomes display properly oriented connexins and enrich the Cx43 expression at cell-cell borders between cardiomyocytes. Through mathematical modeling and experimental validation, we demonstrate that Connectosome incorporation reinforces endogenous gap junctions, promotes synchronous calcium transients, and improves spatial coordination of beating across networks. Mechanistic studies using engineered cell lines with tagged connexin-43 confirm that channel orientation and functionality are critical, supporting a model in which Connectosomes contribute to gap junction coupling. These results show that Connectosomes can synchronize the beating of immature cardiomyocytes by boosting electrochemical communication, laying the groundwork for future therapeutic advances.

Indexed as

Cell CommunicationConnexin 43Gap JunctionsInduced Pluripotent Stem CellsMyocytes, CardiacCell DifferentiationHumansConnexin 43biovesiclescardiomyocyteshiPSCssynchronization

Identifiers

PMID42047747
PMCPMC13271624

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.