ArticleVeterinary world2026
Cryoprotectant-dependent preservation of cellular viability and oocyte-secreted factors in slow-frozen feline ovarian tissue.
Article in Veterinary world, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background and Aim: Cryopreservation of ovarian tissue is an important technique for preserving the reproductive potential of valuable and endangered species and plays a critical role in establishing genetic resource banks. However, the success of ovarian tissue cryopreservation depends largely on the choice of cryoprotective agent (CPA), which must balance protection against intracellular ice formation with potential cytotoxic effects. In feline ovarian tissue, the dense collagen-rich stromal architecture may further limit CPA diffusion, thereby influencing cryopreservation efficiency. This study aimed to evaluate the effects of two commonly used penetrating CPAs, dimethyl sulfoxide (Me Materials and Methods: Ovarian tissues were collected from six healthy domestic cats undergoing routine ovariohys-terectomy. Cortical fragments (approximately 2 × 2 × 3 mm³) were randomly assigned to five groups: Fresh control, Cryo-control (no CPA), 10% Me Results: All cryopreserved groups exhibited significantly higher apoptosis than fresh tissue. However, tissues preserved with Me Conclusion: Me
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