ArticleVeterinary world2026
Dose-dependent effects of mitoquinone on mitochondrial function and post-thaw quality of cryopreserved canine sperm.
Article in Veterinary world, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Comparative Sensitivity of Canine Swim-Up Sperm Fractions Treated with Antifreeze Protein and Trehalose Following Freeze-Thaw.Animals : an open access journal from MDPI · 2026Article
- Effects of Kaempferol Supplementation on the Cryopreservation Quality of Semen from Yuansheng Aite Dairy Rams.Antioxidants (Basel, Switzerland) · 2026Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Background and Aim: Cryopreservation is commonly used in canine reproductive biotechnology to preserve genetic material for long-term storage. However, the freeze-thaw process induces oxidative stress, mitochondrial dysfunction, and structural damage in spermatozoa, thereby reducing post-thaw sperm quality and fertility potential. Mitoquinone (MitoQ), a mitochondria-targeted antioxidant, has been suggested as a promising cryoprotective additive that mitigates mitochondrial reactive oxygen species and enhances cellular integrity. This study aimed to assess the dose-dependent effects of MitoQ supplementation on mitochondrial function, oxidative stress, and the post-thaw quality of cryopreserved canine spermatozoa to identify an optimal concentration for semen cryopreservation. Materials and Methods: Semen samples were collected from 10 healthy and fertile male dogs of various breeds. Each ejaculate was divided into four equal parts and cryopreserved with a Tris-fructose-egg yolk extender supplemented with different MitoQ concentrations (0, 100, 200, or 400 nM). After four weeks of storage in liquid nitrogen, the samples were thawed and evaluated Results: MitoQ supplementation had concentration-dependent effects on several sperm quality parameters. The 200 nM MitoQ group showed the most consistent improvements in post-thaw sperm quality. Total motility and curvilinear velocity were significantly higher at this concentration compared to the control and other treatment groups. Additionally, sperm treated with 200 nM MitoQ displayed improved plasma membrane and acrosome integrity, reduced lipid peroxidation, and a lower proportion of dead sperm cells. Trends toward increased viability and enhanced mitochondrial activity were also seen at this concentration. In contrast, 400 nM MitoQ supplementation was linked to decreased membrane integrity and increased oxidative stress markers, suggesting possible pro-oxidant effects at higher doses. Overall, the results indicate that moderate MitoQ supplementation supports mitochondrial redox balance and partially reduces cryo-induced damage in canine spermatozoa. Conclusion: MitoQ supplementation at 200 nM modestly but consistently enhanced several
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