Evidence map›Paper›PMID 42045204›Full record

ArticleNature communications2026

Quantifying phage-host dynamics using droplet microfluidics.

Louis Givelet, Sophie von Schönberg, Florian Katzmeier, Friedrich C Simmel

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Louis GiveletDepartment of Bioscience, TUM School of Natural Sciences, Technical University Munich, Am Coulombwall 4a, Munich, Germany.ORCID 0000-0003-3900-4239
Sophie von SchönbergDepartment of Bioscience, TUM School of Natural Sciences, Technical University Munich, Am Coulombwall 4a, Munich, Germany.ORCID 0000-0003-4608-1881
Florian KatzmeierDepartment of Bioscience, TUM School of Natural Sciences, Technical University Munich, Am Coulombwall 4a, Munich, Germany.ORCID 0009-0006-2547-3527
Friedrich C SimmelDepartment of Bioscience, TUM School of Natural Sciences, Technical University Munich, Am Coulombwall 4a, Munich, Germany. simmel@tum.de.ORCID 0000-0003-3829-3446

Funding

Bayerisches Staatsministerium für Wirtschaft, Infrastruktur, Verkehr und Technologie (Bavarian Ministry of Economic Affairs, Infrastructure, Transport and Technology) M4-2110-0004EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 813786
6 · The paper itself

Abstract

Since their discovery, bacteriophages-viruses that infect bacteria-have been invaluable to molecular biology and biotechnology. Renewed interest in phage-based antimicrobials, driven by the global antibiotic resistance crisis, highlights the need for improved quantitative tools. While conventional double-layer plaque assays (DLA) have provided foundational insights, they are limited by their inability to monitor infection dynamics over time and the inflexibility in experimental setups. Here, we present a high-throughput droplet microfluidics platform to quantify individual phage infection events. By co-encapsulating individual phages and bacteria in microfluidic droplets, we precisely control key experimental parameters such as exposure time and the ratio of phages to bacteria. This approach enables direct quantification of lysis events and measurement of lysis kinetics without interference from further progeny-driven infection processes inherent to bulk cultures. Applicable to diverse phage-host systems, this method offers a dynamic and accurate framework for studying phage biology and supports the development of phage-based antimicrobial strategies.

Indexed as

BacteriophagesMicrofluidicsEscherichia coliKineticsViral Plaque Assay

Identifiers

PMID42045204
PMCPMC13121708

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.