Evidence map›Paper›PMID 42043722›Full record

ArticleVeterinary research communications2026

Use of peptide nucleic acid fluorescent in situ hybridization technique in the detection of Mycobacterium avium paratuberculosis in fecal samples from dairy herds.

Pallvi Slathia, Deepti Narang, Mudit Chandra, Siddhartha Deshmukh, Leishangthem Geeta Devi, Kuldip Gupta

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Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Pallvi SlathiaDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India.
Deepti NarangDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India. deeptivet@rediffmail.com.
Mudit ChandraDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India.
Siddhartha DeshmukhDepartment of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India.
Leishangthem Geeta DeviDepartment of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India.
Kuldip GuptaDepartment of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141,004, India.

Funding

Department of Biotechnology, Ministry of Science and Technology, India "Mycobacterial diseases in animals network (MyDAN) Programme" (No. BT/AVD/Bovine Tuberculosis/2018)
6 · The paper itself

Abstract

Novel peptide nucleic acid (PNA) probes were designed for direct detection of Mycobacterium avium paratuberculosis (MAP) by the Fluorescent in situ Hybridization technique from fecal samples. For this study, fecal samples (n = 218) were collected from cattle (n = 180) and buffaloes (n = 38) with a history or incidence of cases of chronic intermittent diarrhoea from organized dairy farms in the state of Punjab. Fluorescent in situ Hybridization was done on highly positive (acid-fast positive) fecal samples (n = 54) using a newly designed MAP probe. The designed probe was standardized for the standard culture of Mycobacterium avium subsp. paratuberculosis (ATCC K10) at hybridization conditions of 55 °C and 30% formamide concentration. The probe showed no cross-reactivity with the other Mycobacterial species, viz., M. intracellulare, M. smegmatis, M. fortuitum, and M. vaccae, except Mycobacterium kansasii. However, M. kansasii and Mycobacterium avium subsp. paratuberculosis were clearly differentiated by the probe morphologically. Out of 54 fecal samples, 7 samples tested positive by PNA- FISH which were also tested positive by conventional PCR using the IS900 gene, while the remaining fecal samples were negative by PCR.

Indexed as

Cattle DiseasesFecesIn Situ Hybridization, FluorescenceMycobacterium avium subsp. paratuberculosisParatuberculosisPeptide Nucleic AcidsAnimalsCattleDairyingPeptide Nucleic AcidsFecal samplesFluorescent in situ HybridizationIn-house designed MAP probeMycobacterium avium subsp. paratuberculosis (MAP)

Identifiers

PMID42043722

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.