Host-derived Bacillus velezensis enhances intestinal structure, antioxidant capacity, and pathogen resistance in the American bullfrog (Aquarana catesbeiana).

Yueming Hu, Han Lin, Mengzhen Jiang, Changyi Lin, Hua Li, Jingyang Sun, Kesong Chen, Yebing Yu, Chun Liu

Abstract read

In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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9 authors.

Yueming HuGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Han LinGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Mengzhen JiangGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Changyi LinGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Hua LiGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Jingyang SunGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Kesong ChenGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Yebing YuCollege of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 224051, China.

Chun LiuGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. liuchun@zhku.edu.cn.

Funding

Application Research and Extension of Fermented Chinese Herbal Medicine in Bullfrog Farming under the 2023 Rural Revitalization Strategy KB23Y1101

6 · The paper itself

Abstract

The development of host-derived probiotics presents a forward-looking nutritional component strategy for sustainable aquaculture. However, research on host-associated probiotics for the globally farmed American bullfrog (Aquarana catesbeiana) is limited. The research put Bacillus velezensis (BLS) isolated from bullfrog guts determine its probiotic potential. Bullfrogs were divided into two groups: one fed a control diet and the other fed a diet enriched with 1 × 10⁸ CFU/g BLS for 7 weeks. BLS supplementation significantly enhanced growth performance and markedly decreased mortality after challenge with Streptococcus agalactiae. Relative to controls, the BLS cohort demonstrated heightened activity of essential antioxidant enzymes (CAT, GSH-Px, and SOD) and lower malondialdehyde levels in liver and intestinal tissues. Moreover, BLS supplementation enhanced intestinal structure, demonstrated by markedly increased villus height and goblet cell count, alongside enhanced hepatocyte arrangement and tissue integrity. Gut microbiome analysis revealed that BLS elevated the proportion of Firmicutes while decreasing the proportion of harmful genera such as Elizabethkingia. These findings suggest that the probiotic effects are mediated through the modulation of gut barrier function and microbial community. In conclusion, dietary supplementation with B. velezensis BLS enhanced growth, pathogen resistance, antioxidant capacity, and bowel health in bullfrogs through enhanced intestinal morphology and microbiome regulation. Consequently, supplementation holds significant promise as a probiotic in bullfrog farming.

Indexed as

AntioxidantsBacillusFish DiseasesIntestinesProbioticsRana catesbeianaAnimal FeedAnimalsAquacultureDisease ResistanceStreptococcus agalactiaeAntioxidantsAmerican bullfrogAntioxidant capacityBacillus velezensisIntestinal healthPathogen resistance

Identifiers

PMID42043591

DOI10.1016/j.fsi.2018.11.003

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.