Evidence map›Paper›PMID 42043260›Full record

ArticleViruses2026

Development of a Multiplex PCR Method for Efficient Differential Diagnosis of Clinical Cases and Vaccine Immunization of Marek's Disease.

Wen-Kai Zhang, Man Teng, Lu-Ping Zheng, Bin Shi, Wei-Dong Wang, Gui-Xi Li, Yong-Xu Zhao, Zhen Yang, Zu-Hua Yu, Jun Luo

Abstract read
In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wen-Kai ZhangInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Man TengInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.ORCID 0000-0003-2784-4169
Lu-Ping ZhengInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Bin ShiInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Wei-Dong WangInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Gui-Xi LiShangqiu Center for Animal Quarantine and Disease Control and Prevention, Shangqiu 476100, China.
Yong-Xu ZhaoScientific Support and Investigation Unit, Ceva Animal Health, Hangzhou 310018, China.
Zhen YangJinyu Youbang Biotechnology (Jiangsu) Co., Ltd., Yangzhou 225007, China.
Zu-Hua YuLaboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Jun LuoInstitute for Animal Health & UK-China Centre of Excellence for Research on Avian Diseases, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.ORCID 0000-0002-7351-8180

Funding

Elite Talents Development Program of Henan Academy of Agricultural Sciences 2026RC03National Key Research and Development Program of China 2023YFE0106100Natural Science Foundation of Henan Province 232300421009
6 · The paper itself

Abstract

Marek's disease (MD), caused by pathogenic Marek's disease virus serotype 1 (MDV-1), is one of the most important avian immunosuppressive and neoplastic diseases and has led to huge economic losses to the poultry industry worldwide. Rapid and accurate clinical diagnosis is of great significance for efficient control of the disease. Herein, we have established a multiplex PCR (mPCR) method to simply differentiate all of the three types of MDV, using five specific primers targeting to MDV-1 oncogene meq or MDV-2 and MDV-3/HVT

Indexed as

Herpesvirus 2, GallidMarek DiseaseMarek Disease VaccinesMultiplex Polymerase Chain ReactionPoultry DiseasesAnimalsChickensDiagnosis, DifferentialSensitivity and SpecificityViral VaccinesMarek Disease VaccinesViral Vaccinesdifferential diagnosisMarek’s disease virusmultiplex PCRvaccinationviral infection

Identifiers

PMID42043260
PMCPMC13120613

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.