Evidence map›Paper›PMID 42042695›Full record

ArticleACS chemical biology2026

Site-Specific Immobilization of ZNRF3 Reveals the Importance of Target Structural Integrity on Macrocyclic Peptide Selections.

Demonta D Coleman, Linnette Arceo, Armita Paydar, Alyssa Toner, Arya Kodali, Jacqueline Wright, J Trae Hampton, Wenshe Ray Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Demonta D ColemanTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Linnette ArceoTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Armita PaydarTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Alyssa TonerTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.ORCID 0009-0001-9994-5260
Arya KodaliTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Jacqueline WrightTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
J Trae HamptonTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0001-5562-162X
Wenshe Ray LiuTexas A&M Drug Discovery Center and Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0002-7078-6534

Funding

Use of the Noncanonical Amino Acid Mutagenesis Technique in Combination with Other Approaches to Study Functions of Posttranslational Lysine Modifications in ProteinsR35GM145351 · NIGMS · TEXAS A&M UNIVERSITY · PI Wenshe Ray Liu · 2022 to 2026
$2.6M
A Multiplatform Approach to Develop ENL-Targeting Molecules as Drug Candidates for Acute Myeloid LeukemiaR01CA291968 · NCI · TEXAS A&M UNIVERSITY · PI Wenshe Ray Liu · 2024 to 2026
$2.4M
Development of PD-L1 Proteolysis-Targeting Chimeras (PROTACs): An Innovative Cancer Drug Discovery RouteF31CA268883 · NCI · TEXAS A&M UNIVERSITY · PI COLEMAN, DEMONTA · 2023 to 2025
$117k
NCI NIH HHS F31 CA268883NCI NIH HHS R01 CA291968NIGMS NIH HHS R35 GM145351
6 · The paper itself

Abstract

Phage display of macrocyclic peptide libraries has proven highly effective for ligand discovery, yet the impact of target immobilization and structural integrity on selection outcomes has not been systematically examined. Using the ZNRF3 ectodomain as a model, we incorporated p-azidophenylalanine (AzF) at three phenylalanine residues with distinct solvent exposures (F217, F85, F156) to enable selective perturbation of the protein's structure via site-specific strain-promoted azide-alkyne cycloaddition (SPAAC) immobilization. Biochemical evaluation of the mutants confirmed efficient conjugation and structural disruption of the protein, with the F156AzF mutant displaying the most significant reduction in activity. Phage selections using a CX

Indexed as

Immobilized ProteinsMacrocyclic CompoundsPeptidesAzidesPeptide LibraryPhenylalanine4-azidophenylalanineAzidesImmobilized ProteinsMacrocyclic CompoundsPeptide LibraryPeptidesPhenylalanine

Identifiers

PMID42042695
PMCPMC13184931

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.