Co-Immobilization of SpyTag-Cyclized Enzymes on a γPFD-SpyCatcher Hydrogel to Address Broad Specificity.

Ming-Yue Huang, Qing-Yi Su, Tao Wei, Fu-Xing Niu

Abstract read

In one paragraph

Article in Gels (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Ming-Yue HuangGuangxi Key Laboratory for Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China.

Qing-Yi SuGuangxi Key Laboratory for Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China.

Tao WeiDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou 510640, China.

Fu-Xing NiuGuangxi Key Laboratory for Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China.ORCID 0000-0002-7697-005X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The broad substrate specificity of enzymes, while advantageous for catalytic diversity, often leads to undesired side reactions and reduced product yields in engineered metabolic pathways. To address this challenge, we developed a programmable protein scaffold based on a self-assembled γPFD-SpyCatcher hydrogel for the in vivo co-immobilization of SpyTag-cyclized cascade enzymes, enabling the co-immobilization of cascade enzymes in a spatially organized manner. Enzymes with broad substrate specificities were linearly fused with SpyTags, facilitating their spatial organization on the nanoscaffold within engineered

Indexed as

biosynthesiscoimmobilizationenzyme promiscuitynanoscaffoldSpyTag/SpyCatcher

Identifiers

PMID42042186

DOI10.3390/gels12040348

PMCPMC13116799

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.