A Comprehensive HPLC-HRMS/MS Targeted Screening Method to Detect 90 New Psychoactive Substances in Oral Fluid Samples.

Ilaria Spinella, Fabio Altieri, Simona Pichini, Adele Minutillo, Annagiulia Di Trana

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In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ilaria SpinellaDepartment of Biochemical Sciences "Rossi Fanelli", "La Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Fabio AltieriDepartment of Biochemical Sciences "Rossi Fanelli", "La Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0002-6546-2738

Simona PichiniNational Center on Addiction and Doping, National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy.

Adele MinutilloNational Center on Addiction and Doping, National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy.ORCID 0000-0002-8137-0614

Annagiulia Di TranaNational Center on Addiction and Doping, National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy.ORCID 0000-0002-8456-0827

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The continuous emergence of New Psychoactive Substances (NPS) poses a significant challenge to public health and forensic toxicology due to their unpredictable pharmacology and rapid turnover on the illicit market. This study describes the development and validation of a high-resolution screening method for the simultaneous detection of 90 NPS in oral fluid (OF), a matrix of choice for non-invasive sampling and roadside testing. The analytical workflow utilizes a "dilute-and-shoot" approach (1:2

Indexed as

HPLC-MS/HRMSNew Psychoactive Substancesnitazenesoral fluidscreening methodsynthetic cannabinoidssynthetic cathinones

Identifiers

PMID42041894

DOI10.3390/biology15080616

PMCPMC13113579

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.