Potential role of anaerobic plant-associated bacteria in naphthenic acid degradation.

Plant-associated microorganisms can break down many contaminants, including naphthenic acid fraction compounds (NAFCs)-one of the toxic contaminants in oil sands process-affected water (OSPW). One way to take advantage of these processes is to use constructed wetland treatment systems (CWTSs), where aquatic plants work in coordination with microorganisms to remediate OSPW. However, the dynamics of the microbial communities and the potential contribution of distinct bacteria and fungi inhabiting sediments, rhizosphere, and water that comprise these systems remain poorly understood. To address this, we planted water sedge ( IMPORTANCE: Naphthenic acid fraction compounds (NAFCs) represent one of the most toxic and persistent contaminants in oil sands process-affected water (OSPW), posing a significant environmental challenge. As oil sands operations continue, large volumes of OSPW have accumulated, requiring effective treatment before any potential environmental discharge. Our study explores bioremediation through constructed wetland systems as a sustainable approach to reduce NAFC concentrations. By uncovering the microbial dynamics involved in NAFC degradation, our research provides critical insights into the identification of microbial communities that can enhance the remediation process. This manuscript contributes to advancing the scientific foundation needed to develop effective, biological solutions for OSPW treatment.