Evidence map›Paper›PMID 42040952›Full record

ArticleResearch square2026

Environmentally-induced sperm RNAs shape placentation and fetal growth.

Duane Gischewski Pereira, Raquel Santana da Cruz, Meghali Joshi, Lu Jin, Elaine Chen, Hannah De Los Santos, Lili Rodgers, Caroline Crone, Rong Hu, Andrew D Holmes and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Duane Gischewski PereiraDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Raquel Santana da CruzDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Meghali JoshiDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Lu JinDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Elaine ChenDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Hannah De Los SantosDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Lili RodgersDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Caroline CroneDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Rong HuDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
Andrew D HolmesDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, CA, USA.
Upasna SharmaDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, CA, USA.
Susana GalliDepartment of Biochemistry and Molecular & Cell Biology, Georgetown University, Washington, DC, USA.
Sonia de AssisDepartment of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.ORCID 0000-0001-5053-0614

Funding

Tissue Culture Shared ResourceP30CA051008 · NCI · GEORGETOWN UNIVERSITY · PI MARCUS S NOEL · 1990 to 2026
$71.5M
Paternal DDT exposure and programming of metabolic dysfunction and cancer in offspring: Understanding the role of sperm mirnas and placenta developmentR01ES031611 · NIEHS · GEORGETOWN UNIVERSITY · PI DE ASSIS, SONIA · 2021 to 2025
$2.6M
NCI NIH HHS P30 CA051008NIEHS NIH HHS R01 ES031611
6 · The paper itself

Abstract

Sperm RNAs are environment-sensitive and mediate paternally-induced epigenetic inheritance of traits. The sperm RNA cargo is delivered to the oocyte during fertilization but precise mechanisms by which the 'epigenetic memory' it carries alters embryonic development to shape the progeny phenotypes remains poorly understood. Here, we used two mouse paradigms of male pre-conception experiences to study whether environmentally-induced sperm RNAs regulate placenta development. Using zygotic injections, we show that the sperm RNA load from DDT-treated and obese males impacts placenta function and fetal growth in opposite ways. Mechanistically, these environmentally-driven sperm RNAs disrupt early embryonic development and cell lineage specification. They also dictate the mature placenta cellular composition, nutrient stores, vascularization and imprinted genes landscape. Our findings provide direct evidence that sperm RNAs shape placentation, offering a mechanism by which fathers influence their progeny beyond their shared genome. These data also highlight the role of paternal non-genetic factors in pregnancy outcomes.

Identifiers

PMID42040952
PMCPMC13105136

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.